New insight into the aptamer conformation and aptamer/protein interaction by surface-enhanced Raman scattering and multivariate statistical analysis
| Autor: | Eric Finot, Aymeric Leray, Nadia Djaker, Simion Astilean, Marc Lamy de la Chapelle, Mathieu Edely, Monica Potara, Qiqian Liu, Sarra Gam Derouich, Wafa Safar, Jolanda Spadavecchia, Weiling Fu, Nordin Félidj, Andra-Sorina Tatar |
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| Přispěvatelé: | Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interdisciplinaire Carnot de Bourgogne [Dijon] (LICB), Université de Bourgogne (UB)-Université de Technologie de Belfort-Montbeliard (UTBM)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche Universités Angers-Le Mans-Orléans (ALMOREAL), Université d'Angers (UA)-Université d'Orléans (UO)-Université du Mans |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Surface (mathematics)
[PHYS]Physics [physics] Analyte Chemistry Aptamer [SDV]Life Sciences [q-bio] 02 engineering and technology Biosensing Techniques Aptamers Nucleotide 010402 general chemistry 021001 nanoscience & nanotechnology Spectrum Analysis Raman 01 natural sciences 0104 chemical sciences symbols.namesake symbols Biophysics [CHIM]Chemical Sciences General Materials Science [NLIN]Nonlinear Sciences [physics] Multivariate statistical 0210 nano-technology Raman scattering |
| Zdroj: | Nanoscale Nanoscale, Royal Society of Chemistry, 2021, 13 (29), pp.12443-12453. ⟨10.1039/D1NR02180J⟩ Nanoscale, Royal Society of Chemistry, 2021, 13 (29), pp.12443-12453. ⟨10.1039/d1nr02180j⟩ |
| ISSN: | 2040-3364 2040-3372 |
| Popis: | International audience; We study the interaction between one aptamer and its analyte (the MnSOD protein) by the combination of surface-enhanced Raman scattering and multivariate statistical analysis. We observe the aptamer structure and its evolution during the interaction under different experimental conditions (in air or in buffer). Through the spectral treatment by principal component analysis of a large set of SERS data, we were able to probe the aptamer conformations and orientations relative to the surface assuming that the in-plane nucleoside modes are selectively enhanced. We demonstrate that the aptamer orientation and thus its flexibility rely strongly on the presence of a spacer of 15 thymines and on the experimental conditions with the aptamer lying on the surface in air and standing in the buffer. We reveal for the first time that the interaction with MnSOD induces a large loss of flexibility and freezes the aptamer structure in a single conformation. |
| Databáze: | OpenAIRE |
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