Effect of oxidative stress in rostral ventrolateral medulla on sympathetic hyperactivity after traumatic brain injury
| Autor: | Lijun Hou, Minkun Yu, Jigang Chen, Liquan Lv, Kaiwei Han, Wen Chen, Rongbin Chen, Enbo Qi |
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| Rok vydání: | 2018 |
| Předmět: |
Male
Mean arterial pressure medicine.medical_specialty Sympathetic nervous system Sympathetic Nervous System Traumatic brain injury Hyperkinesis medicine.disease_cause Superoxide dismutase Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Brain Injuries Diffuse Animals Microinjection Psychomotor Agitation 030304 developmental biology 0303 health sciences Medulla Oblongata biology business.industry General Neuroscience Rostral ventrolateral medulla medicine.disease Malondialdehyde Rats Disease Models Animal Oxidative Stress medicine.anatomical_structure Endocrinology chemistry biology.protein business 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | The European journal of neuroscience. 50(2) |
| ISSN: | 1460-9568 |
| Popis: | Sympathetic hyperactivity occurs in a subgroup of patients after traumatic brain injury (TBI). The rostral ventrolateral medulla (RVLM) is a key region for the activity of sympathetic nervous system. Oxidative stress in the RVLM is proved to be responsible for the increased level of sympathetic activity in animal models of hypertension and heart failure. In this study, we investigated whether oxidative stress in the RVLM contributed to the development of sympathetic hyperactivity after TBI in rats. Model of diffuse axonal injury was induced using Sprague-Dawley rats, and level of mean arterial pressure (MAP) and plasma Norepinephrine (NE) was measured to evaluate the sympathetic activity. For the assessment of oxidative stress, expression of reactive oxygen species (ROS), malondialdehyde (MDA), and superoxide dismutase (SOD) in the RVLM was determined. Microinjection of Tempol into the RVLM was performed to determine the effect of oxidative stress on sympathetic hyperactivity. According to the results, TBI led to elevated MAP and plasma NE in rats. It also induced a significantly increased level of ROS, MDA production and decreased level of SOD in the RVLM. The sympathetic activity, ROS, and MDA in the RVLM decreased significantly after microinjection of Tempol. Therefore, the present results suggested that oxidative stress in the RVLM was involved in the development of sympathetic hyperactivity following TBI. |
| Databáze: | OpenAIRE |
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