Development and Validation of an On-Line Water Toxicity Sensor with Immobilized Luminescent Bacteria for On-Line Surface Water Monitoring
| Autor: | Arco J. Wagenvoort, Marjolijn Woutersen, Afua Abrafi Boakye, H.A.M. Ketelaars, Jan Mink, Minne B. Heringa, Robert S. Marks, Bram van der Gaag, Bram Brouwer |
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| Přispěvatelé: | Animal Ecology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Luminescence
Daphnia magna Water monitoring Genetically modified bacteria 010501 environmental sciences medicine.disease_cause lcsh:Chemical technology biosensor 01 natural sciences Biochemistry Article Analytical Chemistry medicine lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation bacteria luminescence genotoxicity water monitoring 0105 earth and related environmental sciences Netherlands Inert biology Bacteria Chemistry Luminescent bacteria 010401 analytical chemistry Water biology.organism_classification Atomic and Molecular Physics and Optics 0104 chemical sciences Environmental chemistry Water quality Genotoxicity SDG 6 - Clean Water and Sanitation Biosensor Surface water Water Pollutants Chemical Environmental Monitoring |
| Zdroj: | Sensors (Basel, Switzerland) Sensors; Volume 17; Issue 11; Pages: 2682 Sensors, Vol 17, Iss 11, p 2682 (2017) Sensors (Switzerland), 17(11):2682. Multidisciplinary Digital Publishing Institute (MDPI) Woutersen, M, Van Der Gaag, B, Boakye, A A, Mink, J, Marks, R S, Wagenvoort, A J, Ketelaars, H A M, Brouwer, B & Heringa, M B 2017, ' Development and validation of an on-linewater toxicity sensor with immobilized luminescent bacteria for surface water monitoring ', Sensors (Switzerland), vol. 17, no. 11, 2682 . https://doi.org/10.3390/s17112682 |
| ISSN: | 1424-8220 |
| Popis: | Surface water used for drinking water production is frequently monitored in The Netherlands using whole organism biomonitors, with for example Daphnia magna or Dreissena mussels, which respond to changes in the water quality. However, not all human-relevant toxic compounds can be detected by these biomonitors. Therefore, a new on-line biosensor has been developed, containing immobilized genetically modified bacteria, which respond to genotoxicity in the water by emitting luminescence. The performance of this sensor was tested under laboratory conditions, as well as under field conditions at a monitoring station along the river Meuse in The Netherlands. The sensor was robust and easy to clean, with inert materials, temperature control and nutrient feed for the reporter organisms. The bacteria were immobilized in sol-gel on either an optical fiber or a glass slide and then continuously exposed to water. Since the glass slide was more sensitive and robust, only this setup was used in the field. The sensor responded to spikes of genotoxic compounds in the water with a minimal detectable concentration of 0.01 mg/L mitomycin C in the laboratory and 0.1 mg/L mitomycin C in the field. With further optimization, which should include a reduction in daily maintenance, the sensor has the potential to become a useful addition to the currently available biomonitors. |
| Databáze: | OpenAIRE |
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