FMRF-amide immunoreactivity in the mammalian gastroenteropancreatic neuroendocrine system
| Autor: | C. L. M. V. J. van Assche, F. J. G. M. Kubben, Fred T. Bosman |
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| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
Pancreatic Polypeptide-Secreting Cells Histology Immunocytochemistry Enteroendocrine cell Pancreatic Polypeptide digestive system Immunoenzyme Techniques Dogs Internal medicine Gastrins medicine Animals Humans Pancreatic polypeptide FMRFamide Pancreas Molecular Biology Gastrin Sheep Chemistry digestive oral and skin physiology Colocalization Cell Biology General Medicine digestive system diseases Rats Medical Laboratory Technology medicine.anatomical_structure Endocrinology Cattle Rabbits Anatomy G cell General Agricultural and Biological Sciences Digestive System Oligopeptides |
| Zdroj: | Histochemistry. 84:439-444 |
| ISSN: | 1432-119X 0301-5564 |
| DOI: | 10.1007/bf00482976 |
| Popis: | The presence of FMRF-amide, a cardioactive tetrapeptide, was studied by immunocytochemistry in human and rat gastric antrum and pancreas, and in the ovine, bovine, canine and rabbit pancreas. In human and rat gastric antrum, numerous cells contained FMRF-amide immunoreactive material. By staining of serial sections and by double staining, colocalization of immunoreactivity for gastrin and FMRF-amide was observed in part of the gastrin cells. In the pancreas of these and the other species, immunoreactivity for FMRF-amide was located both in acinar and islet endocrine cells. Colocalization of FMRF-amide and pancreatic polypeptide was found in a proportion of pancreatic polypeptide cells in the pancreas. FMRF-amide immunoreactivity never colocalized with the other neurohormonal peptides which occur in the gastric antrum and the pancreas. Our observations show that neuroendocrine cells occur in the gastric antrum and pancreas which are exclusively immunoreactive or gastrin and for pancreatic polypeptide respectively. In addition cells occur which show immunoreactivity for FMRF-amide as well as for gastrin in the gastric antrum and with antiserum to FMRF-amide as well as for pancreatic polypeptide in the pancreas. It is concluded that FMRF-amide antibodies probably recognize a substance in G and PP cells which is not identical but may be structurally related to gastrin and pancreatic polypeptide. |
| Databáze: | OpenAIRE |
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