Dye Ligand Epoxide Chitosan/Alginate: A Potential New Stationary Phase for Human IgG Purification
| Autor: | Maria Cristiane Martins de Souza, Diego Romao Gondim, Igor Tadeu Lazzarotto Bresolin, Luana P. Lima, Diana C. S. Azevedo, Wellington S. Adriano, Ivanildo J. Silva |
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| Rok vydání: | 2012 |
| Předmět: |
Chromatography
Elution Ligand General Chemical Engineering lcsh:QD450-801 Epoxide lcsh:Physical and theoretical chemistry Fraction (chemistry) Surfaces and Interfaces General Chemistry Human serum albumin Chitosan chemistry.chemical_compound Adsorption chemistry Affinity chromatography medicine medicine.drug |
| Zdroj: | Adsorption Science & Technology, Vol 30 (2012) |
| ISSN: | 2048-4038 0263-6174 |
| Popis: | Cibacron Blue F3GA immobilized as ligand onto epoxide chitosan/alginate composite was prepared and evaluated for the purification of human immunoglobulin G (IgG) isolated from human serum by dye-ligand affinity chromatography. The effect of buffer pH on IgG adsorption, kinetic and isothern adsorption of high-purity IgG were studied by batch adsorption. The adsorption isotherm data were well fitted (using 25 mmol/L sodium phosphate buffer at pH 6.0) by the Langmuir–Freundlich model with a maximum adsorption capacity of 99 mg/g, apparent dissociation constant of 1.2 × 10 −6 mol/L and n equal to 4.86. Fixed-bed experiments were performed using high-purity IgG, achieving a recovery rate of 53%. Human serum diluted ten times was also injected into the column. An enrichment of IgG in the elution fraction with traces of human serum albumin was observed. According to the study results, this material can be applied in the purification of human IgG under optimized conditions. |
| Databáze: | OpenAIRE |
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