Low‐cost, high‐throughput and rapid‐prototyped 3D‐integrated dielectrophoretic channels for continuous cell enrichment and separation
Autor: | Shabnam A. Faraghat, Henry O. Fatoyinbo, Kai F. Hoettges, Michael Pycraft Hughes |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | ELECTROPHORESIS. |
ISSN: | 1522-2683 0173-0835 |
Popis: | Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor-insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10 000 cells/s. |
Databáze: | OpenAIRE |
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