Low‐cost, high‐throughput and rapid‐prototyped 3D‐integrated dielectrophoretic channels for continuous cell enrichment and separation

Autor: Shabnam A. Faraghat, Henry O. Fatoyinbo, Kai F. Hoettges, Michael Pycraft Hughes
Rok vydání: 2022
Předmět:
Zdroj: ELECTROPHORESIS.
ISSN: 1522-2683
0173-0835
Popis: Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor-insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10 000 cells/s.
Databáze: OpenAIRE