Ubiquitination-mediated interaction among domains is responsible for inhibition of RNA endonuclease activity of mRNA cycling sequence binding protein from L. donovani (LdCSBP)
| Autor: | Dipankar Bhandari, Titash Sen, Kasturi Guha, Partha Saha |
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| Rok vydání: | 2014 |
| Předmět: |
Untranslated region
Paromomycin Protozoan Proteins Biology Endoribonucleases Gene expression Sf9 Cells Transcriptional regulation Animals Protein Interaction Domains and Motifs Enzyme Inhibitors Sequence Deletion Messenger RNA General Veterinary Binding protein Ubiquitination RNA-Binding Proteins RNA Zinc Fingers General Medicine RING finger domain Infectious Diseases Gene Expression Regulation Biochemistry Insect Science Parasitology RNA Cleavage Leishmania donovani |
| Zdroj: | Parasitology Research. 113:2941-2949 |
| ISSN: | 1432-1955 0932-0113 |
| DOI: | 10.1007/s00436-014-3956-z |
| Popis: | In nearly complete absence of transcriptional regulation, messenger RNA (mRNA) turnover mediated through specific cis-elements plays a predominant role in the control of differential gene expression for the disease causing trypanosomatid parasites. In these organisms, the periodic accumulation of S-phase messages during cell cycle is determined by the presence of one or more copies of a conserved CAUAGAAG octanucleotide motif in the untranslated regions of mRNAs. In our previous studies, a multi-domain cycling sequence binding protein LdCSBP from Leishmania donovani was characterized, which binds specifically to the octamer-containing RNAs via its uniquely arranged CCCH-type Zn fingers and degrades them through its small MutS-related (Smr) endonuclease domain, indicative of its potential role in the turnover of the S-phase mRNAs. Interestingly, the protein is modified by the incorporation of a monoubiquitin residue, and the posttranslational modification inhibits its riboendonuclease activity. However, the mechanism of such inhibition was previously unknown. Here, we establish that the CCCH-type Zn finger domain is the site of ubiquitination in LdCSBP and the interaction of CUE domain of the protein with the ubiquitinated Zn finger domain is responsible for inhibition of its riboendonuclease activity. The findings elucidate an inhibitory mechanism of RNA cleavage through ubiquitination-mediated intramolecular interaction among domains of the enzyme. Furthermore, the riboendonuclease activity is inhibited by anti-leishmanial drug paromomycin suggesting that the regulation of RNA metabolism could be a target of the drug. |
| Databáze: | OpenAIRE |
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