Determination of nofedone in human serum by electron-capture GLC
| Autor: | D. Heusse, A. Renard, J. Gregoire, P. Populaire, P. Pasquier |
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| Rok vydání: | 1980 |
| Předmět: |
Detection limit
Heptafluorobutyric anhydride Chromatography Chromatography Gas Indoles Chemical Phenomena Electron capture Chemistry Physical Ethylene Dichloride Aqueous two-phase system Pharmaceutical Science Administration Oral Alcohol Isoindoles Propanolamines Residue (chemistry) chemistry.chemical_compound chemistry Oral administration Injections Intravenous Humans Anti-Arrhythmia Agents |
| Zdroj: | Journal of pharmaceutical sciences. 69(3) |
| ISSN: | 0022-3549 |
| Popis: | An electron-capture GLC method to measure nofedone in human serum was developed. A homolog of nofedone was added to the serum as an internal standard before the sample was alkalinized with pH 9.5 phosphate buffer and extracted with ethylene dichloride containing 0.5% isopentyl alcohol. This organic phase was extracted with 0.2N HCl, the acidic aqueous phase was neutralized immediately, and the extraction with ethylene dichloride was repeated. The ethylene dichloride phase was evaporated to dryness, and the residue was reacted with heptafluorobutyric anhydride. The derivatives were chromatographed at 290 degrees on a 1% Dexsil 300 column. Data on apparent recovery, accuracy, and specificity are given. The detection limit was 5 ng/ml of serum. Serum levels over time in one patient after intravenous administration of 1 mg/kg and after oral administration of 50, 100, and 150 mg of nofedone are presented. |
| Databáze: | OpenAIRE |
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