A Simple and Rapid Chromatographic Strip Test for Detection of Antibody to Porcine Reproductive and Respiratory Syndrome Virus
| Autor: | Sung-Seok Lee, J.-M. Kang, C.-H. Kim, N. K. Shin, Young S. Lyoo, Jung-Hyang Sur, Steven B. Kleiboeker, K.-Y. Jang |
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| Rok vydání: | 2005 |
| Předmět: |
0301 basic medicine
Swine 040301 veterinary sciences viruses animal diseases 030106 microbiology Porcine Reproductive and Respiratory Syndrome Enzyme-Linked Immunosorbent Assay Antibodies Viral Sensitivity and Specificity Serology 0403 veterinary science 03 medical and health sciences Animals Porcine respiratory and reproductive syndrome virus Fluorescent Antibody Technique Indirect Reagent Strips Strip test General Veterinary biology virus diseases 04 agricultural and veterinary sciences Nucleocapsid Proteins Porcine reproductive and respiratory syndrome virus biology.organism_classification Virology Recombinant Proteins biology.protein Female Antibody |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 17:469-473 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/104063870501700512 |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major economic problem for swine industries worldwide despite several disease-reduction strategies such as age-segregated early weaning and all-in-all-out pig movement. Routine diagnosis of PRRSV is carried out by the combined use of an antibody-detecting enzyme-linked immunosorbent assay (ELISA), immunofluorescence, reverse transcription-polymerase chain reaction, and virus isolation. These assays require specialized laboratory equipment in addition to multistep sample handling and sample preparation. The objective of this study was to evaluate a simple pen-side assay (BioSign™ PRRSV) for rapid detection of PRRSV antibody based on a lateral flow chromatographic strip immunoassay system. This assay uses Escherichia coli–expressed viral nucleocapsid protein antigen for detecting antibodies against PRRSV in swine sera. In this report, the authors describe the evaluation of this assay using sera from both clinical samples and experimentally infected piglets. The results were compared with those of a standard, commercially available antibody ELISA (HerdChek®PRRS ELISA) and an indirect immunofluorescence assay using the same serum samples. The BioSign™ PRRSV assay was capable of detecting antibodies in sera known to contain antibodies to PRRSV, resulting in 93.2% sensitivity for samples from experimentally infected pigs and 98.7% sensitivity for clinical serum samples. For sera that did not contain antibodies to PRRSV, the specificity was found to be 98.5% and 99.2% for clinical and experimental serum samples, respectively. |
| Databáze: | OpenAIRE |
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