Neonatal blood lymphocyte subpopulations
| Autor: | G.J.M. Boerma, E.G.E. de Vries, W.M. Comans-Bitter, Pjj Sauer, S. de Bruin-Versteeg, R. de Groot, Fred K. Lotgering, W C J Hop, J. J. M. Van Dongen |
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| Přispěvatelé: | Immunology, Pediatrics, Epidemiology, Clinical Chemistry, Obstetrics & Gynecology, University of Groningen, Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Adult
Lymphocyte B-Lymphocyte Subsets T lymphocytes NK cells Flow cytometry Natural killer cell Immune system Antigen Antigens CD T-Lymphocyte Subsets Receptors Transferrin medicine Humans Lymphocyte Count CORD-BLOOD Erythroid Precursor Cells medicine.diagnostic_test biology flow cytometry FLOW-CYTOMETRY Infant Newborn Antibodies Monoclonal absolute counts T lymphocyte neonates Lymphocyte Subsets Antigens Differentiation B-Lymphocyte Killer Cells Natural medicine.anatomical_structure Cord blood Pediatrics Perinatology and Child Health Immunology CELLS biology.protein Antibody Developmental Biology B lymphocytes |
| Zdroj: | Biology of the Neonate, 77, 230-235. Karger Scopus-Elsevier Biology of the neonate, 77(4), 230-235 |
| ISSN: | 0006-3126 |
| Popis: | We compared the absolute counts of lymphocyte subpopulations in 15 neonates, and 9 adults using the whole lysed blood technique with 15 different triple immunostainings. To obtain accurate absolute lymphocyte counts in neonatal cord blood samples, the flow cytometric 'lympho-gate' was corrected for the erythroid cell contamination by normoblasts and unlysed erythrocytes. In contrast to earlier studies where relative frequencies were reported, we found that the major difference between neonatal and adult lymphocyte subpopulations concerned the much larger pool of naive 'untriggered' cells in neonates, standby for participation in primary immune responses. Copyright (C) 2000 S. Karger AG, Basel. |
| Databáze: | OpenAIRE |
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