Age-related variation in the cellular composition of equine bronchoalveolar lavage fluid

Autor: Jessica M. Gilbertie, Sandra K. Clark, Doug E. Jones, Brett A. Sponseller, Sarah A. Wiechert, Shannon J. Hostetter
Rok vydání: 2017
Předmět:
Zdroj: Veterinary clinical pathology. 46(2)
ISSN: 1939-165X
Popis: Background Previous reports reveal variation in the cellular composition of equine bronchoalveolar lavage fluid (BALF). Objectives The purpose of this study was to compare the profiles of BALF from horses to assess age-related differences. Serial BALF samples were collected from the same individuals over a one-year period to identify changes in individual animals as they aged. Methods Collection of BALF was performed on horses aged one week and one, 2, 6, and 12 months. Total nucleated cell count (TNCC), protein concentration, and cytology were assessed. Longitudinal analysis was performed and compared to healthy adults. Results Foals at one week and 6 months of age had significantly higher TNCC than adults (medians: 320/μL, 285/μL, and 90/μL, respectively); no differences in total protein were found. Foals at one month had the highest proportion of macrophages (median: 87.3%), differing significantly from both yearlings and adults (medians: 45.5% and 48.7%, respectively). Foals aged one week and one month had significantly lower proportions of lymphocytes than yearlings and adults (medians: 3.2% and 4.7% vs 43.2% and 45.8%, respectively). Eosinophil percentage was lowest in foals aged one week, one month, and 2 months (median: 0.0%) and highest in foals aged 6 months (median: 2.2%). Mast cell percentages were highest in yearlings and adults (medians: 2.2% and 3.3%, respectively) and neutrophil percentage was highest in foals aged one week (13.7%). Conclusions Cytologic profiles of BALF from foals and adult horses differed considerably. Significant changes in TNCC and percentages of lymphocytes, macrophages, and eosinophils occurred with age.
Databáze: OpenAIRE
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