Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite
| Autor: | Yongliang Guo, Wen-E Liu, Liu Lu, Qi Weinan, Guofan Wu, Jie Du, Xiaoquan Lu |
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| Rok vydání: | 2018 |
| Předmět: |
Nanocomposite
Graphene 010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Electrochemical gas sensor Nanomaterials Catalysis law.invention chemistry.chemical_compound Blue colored chemistry law Nitrite 0210 nano-technology Biosensor Nuclear chemistry |
| Zdroj: | Analytical and bioanalytical chemistry. 411(10) |
| ISSN: | 1618-2650 |
| Popis: | Enzyme-mimicking (nanozyme)-based biosensors are attractive owing to their unique catalytic efficiency, multifunctionality, and tunable activity, but examples of oxidase-like nanozymes are quite rare. Herein, we demonstrated that histidine-capped gold nanoclusters (His@AuNCs) possessed intrinsic oxidase-like activity, which could directly oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to blue colored ox-TMB without H2O2. The assembly of reduced graphene oxide (RGO) with His@AuNCs could further improve its oxidase-like activity and the His@AuNCs/RGO nanocomposites had a lower Michaelis constant (Km) and higher catalytic constant (Kcat) for TMB oxidation. Furthermore, compared to other nanomaterials, the as-prepared His@AuNCs/RGO also exhibited enhanced electrocatalytic activity toward TMB. Interestingly, nitrite inhibited the catalytic (chromogenic) and electrocatalytic processes of His@AuNCs/RGO in the oxidation of TMB. The oxidase-like and electrocatalytic activity of His@AuNCs/RGO was evaluated with nitrite and TMB as substrates, and the results indicated that TMB and nitrite might share the same catalytic active sites. On the basis of these findings, a colorimetric and electrochemical sensor was developed with the His@AuNCs/RGO composite as an oxidase mimic for determination of nitrite with linear ranges of 10–500 μM and 2.5–5700 μM, respectively. The developed method was successfully applied to the detection of nitrites in real samples. The present work suggests that the oxidase-like nanozyme is not only suitable for colorimetric assay but also for development of electrochemical sensors in bioanalysis. |
| Databáze: | OpenAIRE |
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