Kinetics modeling of the acidolysis with immobilized Rhizomucor miehei lipases for production of structured lipids from sunflower oil
| Autor: | Maria Elena Carrin, Camila Andrea Palla |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Environmental Engineering
food.ingredient Glyceride Biomedical Engineering Rhizomucor miehei Bioengineering INGENIERÍAS Y TECNOLOGÍAS Reaction intermediate KINETIC PARAMETERS Biotecnología Industrial Palmitic acid chemistry.chemical_compound ENZYME BIOCATALYSIS food MODELING Organic chemistry Lipase Chromatography biology Chemistry Sunflower oil Bioprocesamiento Tecnológico Biocatálisis Fermentación biology.organism_classification Hexane biology.protein LIPASE Stearic acid Biotechnology |
| Zdroj: | Biochemical Engineering Journal. 90:184-194 |
| ISSN: | 1369-703X |
| DOI: | 10.1016/j.bej.2014.06.006 |
| Popis: | Sunflower oil modification for production of semisolid fats was carried out via acidolysis using palmitic and stearic acids (P+St), hexane and a developed biocatalyst from Rhizomucor mieheilipases. Its kinetic behavior was studied by employing three mathematical models proposed in the literature. Furthermore, a new model was proposed to describe not only the variation of triacylglycerols (TAG), diacylglycerols (DAG), and free fatty acids groups but also the acyl migration reaction occurrence. The effect of the reaction temperature on the kinetic and equilibrium parameters, as well as TAG and reaction intermediates profiles was analyzed. Increasing reaction temperature generated major changes in the overall composition of acylglycerols and gave rise to the highest composition of P+St in the obtained structured lipids (58%, 70 h, 60 °C). P+St incorporation was successfully adjusted by an empirical model (Model I) and a lumped parameter model (Model II) for all the studied reaction times, while the model based on a Ping Pong Bi Bi mechanism (Model III) was only able to describe the kinetics behavior (through the variation of reactant saturated fatty acids concentration) until 24 h. Experimental data were fit satisfactorily by the proposed model (Model IV), showing that the increment in the disaturated TAG formation achieved by the increment in temperature was principally related to the favored DAG formation from triunsaturated TAG. Fil: Palla, Camila Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina Fil: Carrin, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect