Signaling and metabolic properties of fast and slow smooth muscle types from mice
| Autor: | Ferenc Szekeres, Anders Arner, Lena Boberg |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Myosin isoforms Muscle Physiology Fysiologi Physiology Glucose uptake Clinical Biochemistry AMP-Activated Protein Kinases Mice 03 medical and health sciences chemistry.chemical_compound Contractile kinetics 0302 clinical medicine AMP-activated protein kinase Physiology (medical) Myosin Animals Protein Isoforms Glycolysis Aorta Shortening velocity Lipoprotein lipase Hexokinase Myosin Heavy Chains biology Chemistry Tonic Gluconeogenesis Glucose transporter Muscle Smooth Energy metabolism Lipid Metabolism Up-Regulation Phasic Cell biology Mice Inbred C57BL Glucose 030104 developmental biology biology.protein Calcium Female 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Pflugers Archiv |
| ISSN: | 1432-2013 0031-6768 |
| DOI: | 10.1007/s00424-017-2096-6 |
| Popis: | This study aims to improve the classification of smooth muscle types to better understand their normal and pathological functional phenotypes. Four different smooth muscle tissues (aorta, muscular arteries, intestine, urinary bladder) with a 5-fold difference in maximal shortening velocity were obtained from mice and classified according to expression of the inserted myosin heavy chain (SMHC-B). Western blotting and quantitative PCR analyses were used to determine 15 metabolic and 8 cell signaling key components in each tissue. The slow muscle type (aorta) with a 12 times lower SMHC-B had 6-fold lower expression of the phosphatase subunit MYPT1, a 7-fold higher expression of Rhokinase 1, and a 3-fold higher expression of the PKC target CPI17, compared to the faster (urinary bladder) smooth muscle. The slow muscle had higher expression of components involved in glucose uptake and glycolysis (type 1 glucose transporter, 3 times; hexokinase, 13 times) and in gluconeogenesis (phosphoenolpyruvate carboxykinase, 43 times), but lower expression of the metabolic sensing AMP-activated kinase, alpha 2 isoform (5 times). The slow type also had higher expression of enzymes involved in lipid metabolism (hormone-sensitive lipase, 10 times; lipoprotein lipase, 13 times; fatty acid synthase, 6 times; type 2 acetyl-coenzyme A carboxylase, 8 times). We present a refined division of smooth muscle into muscle types based on the analysis of contractile, metabolic, and signaling components. Slow compared to fast smooth muscle has a lower expression of the deactivating phosphatase and upregulated Ca2+ sensitizing pathways and is more adapted for sustained glucose and lipid metabolism. © 2018 The Author(s) Correspondence Address: Arner, A.; Department of Physiology and Pharmacology, Karolinska Institutet, v Eulers v 8, Sweden; email: Anders.Arner@ki.seCC BY 4.0 |
| Databáze: | OpenAIRE |
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