Hepatitis B virus (HBV)-specific in vitro antibody production by peripheral blood mononuclear cells (PBMC) after vaccination by recombinant hepatitis B surface antigen (rHBsAg)
| Autor: | A M Bianchi-Mondain, J.P. Vendrell, R Poncet, G Pageaux, J. Ducos, A. Serre, Michel Segondy, Anne-Marie Conge |
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| Rok vydání: | 1996 |
| Předmět: |
Adult
Male HBsAg Hepatitis B vaccine Time Factors Immunology Review medicine.disease_cause Lymphocyte Activation Peripheral blood mononuclear cell Immune system Immunology and Allergy Medicine Humans Hepatitis B Vaccines Hepatitis B Antibodies Cells Cultured Erythrocyte rosetting Hepatitis B virus Vaccines Synthetic Hepatitis B Surface Antigens biology business.industry virus diseases biology.organism_classification Virology digestive system diseases Recombinant Proteins Hepadnaviridae biology.protein Leukocytes Mononuclear Female Antibody business |
| Zdroj: | Clinical and experimental immunology. 103(1) |
| ISSN: | 0009-9104 |
| Popis: | SUMMARY To study the immunization induced by rHBsAg, we analysed the in vitro antibody production (IVAP) to HBsAg by PBMC from 18 subjects vaccinated by two injections on days 0 and 30. HBsAg-specific IVAP was detectable in all subjects after both the first and the second injection, and lasted for about 10 days and then disappeared. However, when the spontaneous HBsAg-specific IVAP became negative, HBsAg stimulation of PBMC cultures induced again a specific HBsAg IVAP. Cultures of cell populations separated by erythrocyte rosetting or Percoll density centrifugation showed that the cells responsible for spontaneous secretion, after in vivo stimulation, were low-density B lymphocytes. High-density B lymphocytes were involved in anti-HBs production induced by in vitro stimulation when spontaneous secretion disappeared. These data suggest that the IVAP test could be a source of important information along with serologic analysis for exploration of the immune response to hepatitis B vaccine. |
| Databáze: | OpenAIRE |
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