Perinatal exposure to glyphosate or a glyphosate-based formulation disrupts hormonal and uterine milieu during the receptive state in rats

Autor: Virginia Lorenz, Maria Mercedes Milesi, Enrique H. Luque, Guillermina Pacini, Jorgelina Varayoud
Rok vydání: 2020
Předmět:
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
animal structures
Glycine
Uterus
ESTROGEN RECEPTOR ALPHA
GLYPHOSATE
Biology
Toxicology
UTERUS
03 medical and health sciences
0404 agricultural biotechnology
Estrus
Pregnancy
Internal medicine
Progesterone receptor
medicine
Animals
Weaning
Embryo Implantation
030304 developmental biology
17Β-ESTRADIOL
0303 health sciences
integumentary system
Perinatal Exposure
Herbicides
Reproduction
04 agricultural and veterinary sciences
General Medicine
Bioquímica y Biología Molecular
040401 food science
Hormones
Rats
Medicina Básica
medicine.anatomical_structure
Endocrinology
Animals
Newborn
Gene Expression Regulation
Sex steroid
Prenatal Exposure Delayed Effects
embryonic structures
IMPLANTATION-RELATED GENES
Gestation
Female
IMPLANTATION
Estrogen receptor alpha
Food Science
Hormone
Zdroj: Food and Chemical Toxicology. 143:111560
ISSN: 0278-6915
DOI: 10.1016/j.fct.2020.111560
Popis: We investigated the effects of perinatal exposure to a glyphosate-based herbicide (GBH) or glyphosate alone (Gly) on female fertility and the hormonal and uterine milieu during the preimplantation period. F0 pregnant rats orally received a GBH or Gly in a dose of 2 mg of glyphosate/kg/day from gestational day (GD) 9 until weaning. F1 females were evaluated to determine the reproductive performance on GD19; and the sex steroid serum levels, the expression of estrogen receptor alpha (ERα), progesterone receptor (PR) and implantation-related genes on GD5 (preimplantation period). GBH and Gly induced preimplantation losses in F1 rats. GBH and Gly groups exhibited higher 17β-estradiol serum levels, without changes in progesterone. Both compounds increased the uterine ERα protein expression, with no differences at transcript level; and only Gly decreased PR mRNA expression. Also, GBH and Gly downregulated Hoxa10 and Lif genes, with no difference in Muc1 and Areg expression. To conclude, perinatal exposure to a GBH or Gly disrupted critical hormonal and uterine molecular targets during the receptive state, possibly associated with the implantation failures. Overall, similar results were found in GBH- and Gly-exposed rats, suggesting that the active principle might be the main responsible for the deleterious effects. Fil: Lorenz, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Pacini, Guillermina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Milesi, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Databáze: OpenAIRE
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