Pathway of Glycine Betaine Biosynthesis in Aspergillus fumigatus
| Autor: | Rui Tada, Isabel Valsecchi, Giovanni Gadda, Andrea Pennati, Stephen Sherman, Hajime Sato, Jean-Paul Latgé, Karine Lambou, Rémi Beau |
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| Přispěvatelé: | Aspergillus, Institut Pasteur [Paris], Georgia State University, University System of Georgia (USG), Bruker Biospin KK [Japan], This study was supported in part by NSF-CAREER grant MCB-0545712 and NSF grant MCB-1121695 (G.G.) and European grants ALLFUNFP7260338 and ESF Fuminomics RNP 06-132 (J.-P.L.)., European Project: 260338,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,ALLFUN(2010), Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Aspergillus fumigatus Choline chemistry.chemical_compound Betaine MESH: Fungal Proteins/metabolism [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Gene Expression Regulation Fungal MESH: Betaine/analogs & derivatives [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology 0303 health sciences Fungal protein MESH: Kinetics Fungal genetics MESH: Flavin-Adenine Dinucleotide/metabolism General Medicine Choline oxidase Articles Spores Fungal MESH: Spores Fungal/genetics [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biochemistry Flavin-Adenine Dinucleotide Betaine-aldehyde dehydrogenase MESH: Fungal Proteins/genetics MESH: Betaine-Aldehyde Dehydrogenase/genetics MESH: Gene Expression Regulation Fungal MESH: Spores Fungal/metabolism MESH: Mutation Betaine-Aldehyde Dehydrogenase Biology Microbiology Cofactor MESH: Mycelium/metabolism Fungal Proteins 03 medical and health sciences MESH: Alcohol Oxidoreductases/genetics Species Specificity MESH: Enzyme Assays MESH: Species Specificity MESH: Aspergillus fumigatus/genetics MESH: Aspergillus fumigatus/metabolism Molecular Biology MESH: Betaine/metabolism 030304 developmental biology Enzyme Assays Mycelium 030306 microbiology MESH: Alcohol Oxidoreductases/metabolism MESH: Choline/metabolism biology.organism_classification MESH: Mycelium/genetics Alcohol Oxidoreductases Kinetics chemistry MESH: Betaine-Aldehyde Dehydrogenase/metabolism Glycine Mutation biology.protein [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Eukaryotic Cell Eukaryotic Cell, American Society for Microbiology, 2013, 12 (6), pp.853-863. ⟨10.1128/EC.00348-12⟩ Eukaryotic Cell, 2013, 12 (6), pp.853-863. ⟨10.1128/EC.00348-12⟩ |
| ISSN: | 1535-9778 1535-9786 |
| Popis: | The choline oxidase ( CHOA ) and betaine aldehyde dehydrogenase ( BADH ) genes identified in Aspergillus fumigatus are present as a cluster specific for fungal genomes. Biochemical and molecular analyses of this cluster showed that it has very specific biochemical and functional features that make it unique and different from its plant and bacterial homologs. A. fumigatus ChoAp catalyzed the oxidation of choline to glycine betaine with betaine aldehyde as an intermediate and reduced molecular oxygen to hydrogen peroxide using FAD as a cofactor. A. fumigatus Badhp oxidized betaine aldehyde to glycine betaine with reduction of NAD + to NADH. Analysis of the AfchoA Δ:: HPH and AfbadA Δ:: HPH single mutants and the AfchoA Δ AfbadA Δ:: HPH double mutant showed that Af ChoAp is essential for the use of choline as the sole nitrogen, carbon, or carbon and nitrogen source during the germination process. Af ChoAp and Af BadAp were localized in the cytosol of germinating conidia and mycelia but were absent from resting conidia. Characterization of the mutant phenotypes showed that glycine betaine in A. fumigatus functions exclusively as a metabolic intermediate in the catabolism of choline and not as a stress protectant. This study in A. fumigatus is the first molecular, cellular, and biochemical characterization of the glycine betaine biosynthetic pathway in the fungal kingdom. |
| Databáze: | OpenAIRE |
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