Different Osteogenic Potentials of Recombinant Human BMP-6 Adeno-Associated Virus and Adenovirus in Two Rat Strains
Autor: | El Noh, Ann-Shung Lieu, Gregory A. Helm, Gerald R. Hankins, Lindsey Jacobson, Jin Zhong Li, Debra D. Pittman, Hongwei Li, John A. Chiorini |
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Rok vydání: | 2006 |
Předmět: |
CD4-Positive T-Lymphocytes
Pathology medicine.medical_specialty Time Factors Viral antibody Bone Morphogenetic Protein 6 Genetic Vectors Computed tomography CD8-Positive T-Lymphocytes medicine.disease_cause Ectopic bone formation Adenoviridae law.invention Rats Sprague-Dawley Rats Nude Immune system Species Specificity Osteogenesis law Internal medicine medicine Animals Humans Bone formation Muscle Skeletal Adeno-associated virus medicine.diagnostic_test Tomography X-Ray business.industry General Engineering Dependovirus Recombinant Proteins Rats Bone morphogenetic protein 6 Endocrinology Bone Morphogenetic Proteins Recombinant DNA business |
Zdroj: | Tissue Engineering. 12:209-219 |
ISSN: | 1557-8690 1076-3279 |
Popis: | The osteogenic potential of AAV5hBMP6 was compared with that of ADhBMP6 in immunodeficient and immunocompetent rats. AAV5hBMP6 (2.3 x 10(12) particles) and ADhBMP6 (5 x 10(7) PFU) elicited viral antibody production in immunocompetent rats. Among rats that received AAV5hBMP6, the earliest time points at which the bone was visible under CT scanner were 30 days in 2-month-old Sprague-Dawley (SD) rats and 60 days in 18-month-old SD rats. The mean volumes of ectopic bone 90 days after viral injection were 0.31 +/- 0.14 cm(3) in athymic nude rats, 0.64 +/- 0.12 cm(3) in 2-month-old SD rats, and 0.21 +/- 0.10 cm(3) in 18-month-old SD rats. In contrast, among rats that received ADhBMP6, the earliest time points to observe the bone formation by CT scan were 15 days in 2-month-old rats and no bone formation in 18-month-old SD rats. The mean volumes of ectopic bone were 4.17 +/- 0.05 cm(3) in athymic nude rats and 0.06 +/- 0.03 cm(3) in 2-month-old SD rats. Although both types of viruses induced an immune response in immunocompetent animals, this response played different roles in the process of bone formation induced by the BMP6 vectors. |
Databáze: | OpenAIRE |
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