Application of high-mannose-type glycan-specific lectin from Oscillatoria Agardhii for affinity isolation of tumor-derived extracellular vesicles
| Autor: | Tomoko Fukushige, Takuro Kanekura, Takehiro Suzuki, Kanji Hori, Mika Yamamoto, Yoichiro Harada, Naoshi Dohmae, Ikuro Maruyama, Munekazu Yamakuchi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Glycan
ADAM10 Biophysics 01 natural sciences Biochemistry 03 medical and health sciences Extracellular Vesicles Agglutinin Bacterial Proteins Polysaccharides Disintegrin medicine Human Umbilical Vein Endothelial Cells Humans Secretion Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Metalloproteinase biology Chemistry Melanoma 010401 analytical chemistry Cell Biology Fibroblasts medicine.disease HCT116 Cells 0104 chemical sciences Neoplasm Proteins Mannose-Binding Lectins A549 Cells Oscillatoria biology.protein Glycoprotein Protein Binding |
| Zdroj: | Analytical biochemistry. 580 |
| ISSN: | 1096-0309 |
| Popis: | Tumor cells secrete membrane vesicles of various sizes, termed extracellular vesicles (EVs), which have gained increasing attention as potential tumor diagnostic markers. Tumor-derived EVs are enriched with high-mannose-type glycans. Here, we report the affinity isolation of EVs from human melanoma A375 cells by using high-mannose-type glycan-specific agglutinin from Oscillatoria Agardhii (OAA). Glycan analysis of melanoma EVs revealed the presence of high-mannose-type glycans with structural units preferred by OAA. We showed that in solution, OAA binds to melanoma EVs in a high-mannose-type glycan-dependent manner. Furthermore, OAA-immobilized beads were found to capture 60% of the particles and most proteinous components from melanoma EVs. Major EV glycoproteins that potentially interact with OAA were identified to be cluster of differentiation 109 (CD109), integrin α6 and a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10). In addition to melanoma EVs, OAA captured EVs from human lung cancer, glioblastoma and colon cancer cells, but not those from endothelial cells and fibroblasts. These results indicate that OAA-immobilized beads may serve as a novel platform for affinity-capture of tumor-derived EVs. |
| Databáze: | OpenAIRE |
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