Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria
| Autor: | Iara J. de Messias-Reason, Segun I. Oyedeji, Imad Faik, Peter G. Kremsner, Zulkarnain Md Idris, Jürgen F. J. Kun, Bertrand Lell |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Linkage disequilibrium Immunology Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Linkage Disequilibrium law.invention Gene Frequency law Lectins TaqMan Immunology and Allergy Humans Allele Child Promoter Regions Genetic Polymerase chain reaction Genetic Association Studies Genetics Polymorphism Genetic Haplotype Infant Promoter General Medicine Molecular biology Malaria Real-time polymerase chain reaction Haplotypes Child Preschool Female |
| Zdroj: | Human immunology. 72(1) |
| ISSN: | 1879-1166 |
| Popis: | Human ficolin-2 (L-ficolin; FCN2) is a serum protein binding to sugar moieties of different human micro-pathogens forcing phagocytosis. Here, we investigate the clinical significance of FCN2 in African children with either mild or severe malaria (n = 130 and n = 108, respectively) from Gabon by analyzing three promoter SNPs (-986G>A, -602G>A, and -4A>G) and one single nucleotide polymorphisms (SNP) in exon 8 (+6424G>T) using quantitative TaqMan, real-time polymerase chain reaction (PCR). In addition, we measured the ficolin-2 plasma levels at two time points: on admission (t(0), acute disease) and 4 weeks after treatment (t(1), healthy phase). Comparison of ficolin-2 plasma levels shows that ficolin-2 concentration is highest during acute severe disease. In addition, we determined polymorphisms in the promoter and all coding regions of FCN2 in 40 Gabonese. Linkage disequilibrium data revealed polymorphic allelic combination patterns in the FCN2 promoter region; strong allelic combinations at -986 and -4, and -557 and -64 were found. No FCN2 promoter haplotypes were significantly distributed between mild and severe cases. |
| Databáze: | OpenAIRE |
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