Aneugenicity and clastogenicity in freshwater fish Oreochromis niloticus exposed to incipient safe concentration of tannery effluent
| Autor: | H.S. Patil, Melaku Gizaw, Mekibib Dawit, Daniel Abera, Moa Abate, Abel Weldetinsae, Esayas Alemayehu, Abebe Getahun |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromium Gills Erythrocytes Health Toxicology and Mutagenesis Industrial Waste 010501 environmental sciences Biology medicine.disease_cause Kidney 01 natural sciences Toxicology 03 medical and health sciences Clastogen medicine Bioassay Animals Food science Effluent 0105 earth and related environmental sciences Cell Nucleus Micronucleus Tests Public Health Environmental and Occupational Health Tanning General Medicine Cichlids Contamination biology.organism_classification Aneugens Pollution Oreochromis 030104 developmental biology Micronucleus test Toxicity Genotoxicity Water Pollutants Chemical DNA Damage Mutagens |
| Zdroj: | Ecotoxicology and environmental safety. 138 |
| ISSN: | 1090-2414 |
| Popis: | Conventional effluent bioassays mostly rely on overt responses or endpoints such as apical and Darwinian fitness. Beyond the empirical observation, laboratory toxicity testing needs to rely on effective detection of prognostic biomarkers such as genotoxicity. Indeed, characterization of tannery effluent requires slotting in of genotoxic responses in whole effluent toxicity testing procedures. Hence, the prime objective of the present experimental investigation is to apply the technique of biological assay as a tool of toxicity testing to evaluate the induction of micronuclei (MN) in peripheral erythrocytes, and exfoliated cells of gill and kidney of O.niloticus exposed to Maximum tolerable concentrations (MTCs) of composite Modjo tannery effluent (CMTE) and to compare the sensitivity of each cells origin to the induction of MN. After 72h of exposure, cellular aberrations were detected using MN and nuclear abnormality (NA) tests. The induction of MN was significantly higher in exposed groups (P0.05) when compared to the control group; moreover the tissue specific MN response was in the order, gill cellsperipheral erythrocytekidney. Total NA was found to increase significantly (P0.05), when compared to the non-exposed group. NA was also further ramified as blebbed (BL), bi-nucleated (BN), lobbed (LB) and notched (NT) abnormalities. The result of each endpoint measured has demonstrated that at a concentration of total chromium (0.1, 0.73 and 1.27mg/L), a perceptible amount cellular aberration was measured, further implicating somber treat of genotoxicity to fishes, if exposed to water contaminated with tannery effluent. This further highlight that conventional effluent monitoring alone cannot reveal the effects expressed at cellular and genetic levels further demanding the incorporation of effluent bioassays in risk assessment and risk management/abatement programs. |
| Databáze: | OpenAIRE |
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