Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis
| Autor: | Per Kragh Andersen, Teis Esben Sondergaard, Peter Funch, Jakob Tophøj, Niels Thomas Eriksen, Rasmus Dam Wollenberg |
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| Rok vydání: | 2019 |
| Předmět: |
Water microbiology
0106 biological sciences 0301 basic medicine Cell kinetics Cell division Denmark Cell lcsh:Medicine Biology 010603 evolutionary biology 01 natural sciences Article 03 medical and health sciences Species Specificity Botany medicine Seawater Diaphanoeca grandis SDG 14 - Life Below Water Life history lcsh:Science Choanoflagellate Pantoea sp Choanoflagellata Biological Phenomena Marine biology Multidisciplinary Pantoea lcsh:R biology.organism_classification Kinetics 030104 developmental biology medicine.anatomical_structure lcsh:Q Single-Cell Analysis Lorica (biology) Cell Division |
| Zdroj: | Eriksen, N T, Wollenberg, R D, Tophøj, J, Sondergaard, T E, Funch, P & Andersen, P 2019, ' Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis ', Scientific Reports, vol. 9, no. 1, 14543 . https://doi.org/10.1038/s41598-019-50998-0 Scientific Reports Eriksen, N T, Tophøj, J, Wollenberg, R D, Sondergaard, T E, Funch, P & Andersen, P 2019, ' Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis ', Scientific Reports, vol. 9, no. 1, 14543, pp. 1 . https://doi.org/10.1038/s41598-019-50998-0 Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Choanoflagellates are common members of planktonic communities. Some have complex life histories that involve transitions between multiple cell stages. We have grown the loricate choanoflagellate Diaphanoeca grandis on the bacterium Pantoea sp. and integrated kinetic observations at the culture level and at the single cell level. The life history of D. grandis includes a cell division cycle with a number of recognisable cell stages. Mature, loricate D. grandis were immobile and settled on the bottom substratum. Daughter cells were ejected from the lorica 30 min. after cell division, became motile and glided on the bottom substratum until they assembled a lorica. Single cell kinetics could explain overall growth kinetics in D. grandis cultures. The specific growth rate was 0.72 day−1 during exponential growth while mature D. grandis produced daughter cells at a rate of 0.9 day−1. Daughter cells took about 1.2 h to mature. D. grandis was able to abandon and replace its lorica, an event that delayed daughter cell formation by more than 2 days. The frequency of daughter cell formation varied considerably among individuals and single cell kinetics demonstrated an extensive degree of heterogeneity in D. grandis cultures, also when growth appeared to be balanced. |
| Databáze: | OpenAIRE |
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