Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement
| Autor: | Zbigniew A. Janowicz, Merckelbach Armin J, Cornelis P. Hollenberg, Stefanie Gödecke |
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| Rok vydání: | 1993 |
| Předmět: |
Genetic Markers
Genetics biology Genes Fungal Orotidine-5'-Phosphate Decarboxylase Saccharomyces cerevisiae Mutant Fungal genetics food and beverages Locus (genetics) Sequence Analysis DNA General Medicine Molecular cloning biology.organism_classification Applied Microbiology and Biotechnology Saccharomyces Molecular biology Pichia Yeast Transformation Genetic Mutagenesis Site-Directed URA3 Cloning Molecular Gene Deletion Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 40 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/bf00170393 |
| Popis: | The ura3 gene of Hansenula polymorpha was cloned, sequenced and used to generate a ura3 mutant from the wild-type strain of this yeast via integrative mutagenesis. The Tn5 neomycin-resistance marker (neo) under control of the ADH1 promoter from Saccharomyces cerevisiae served as a transformation marker. The results show that gene replacement can be achieved in H. polymorpha, a yeast with a high level of non-homologous integration. |
| Databáze: | OpenAIRE |
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