Biochemical and molecular characterization of sialylated cervical mucins in sheep
| Autor: | Laura Abril-Parreño, Jack Morgan, Anette Krogenæs, Xavier Druart, Paul Cormican, Mary E Gallagher, Colm Reid, Kieran Meade, Radka Saldova, Sean Fair |
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| Přispěvatelé: | University of Limerick (UL), Teagasc - The Agriculture and Food Development Authority (Teagasc), The National Institute for Bioprocessing Research and Training, Norwegian University of Life Sciences (NMBU), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University College Dublin [Dublin] (UCD), National University of Ireland [Galway] (NUI Galway), ANR-16-SUSN-0001,SusSheP,Sustainable Sheep Production(2016), European Project: 696231,H2020,H2020-ISIB-2015-1,SusAn(2016) |
| Rok vydání: | 2022 |
| Předmět: |
Male
fertility [SDV.SA]Life Sciences [q-bio]/Agricultural sciences Sheep Pregnancy Rate cervical mucus sperm transport [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Cell Biology General Medicine N-Acetylneuraminic Acid Reproductive Medicine Pregnancy Semen sialic acid ewe breed Animals Female estrus cycle Insemination Artificial Semen Preservation |
| Zdroj: | Biology of Reproduction Biology of Reproduction, 2022, 107 (2), pp.419-431. ⟨10.1093/biolre/ioac077⟩ |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1093/biolre/ioac077 |
| Popis: | Sialic acid occupies terminal positions on O-glycans of cervical mucins, where they contribute to the increased viscosity of mucin thereby regulating sperm transport. This study characterized the sialylated cervical mucins from follicular phase mucus of six European ewe breeds with known differences in pregnancy rates following cervical artificial insemination (AI) using frozen–thawed semen at both synchronized and natural estrus cycles. These were Suffolk (low fertility) and Belclare (medium fertility) in Ireland, Ile de France and Romanov (both with medium fertility) in France, and Norwegian White Sheep (NWS) and Fur (both with high fertility) in Norway. Expression of mucin and sialic acid related genes was quantified using RNA-sequencing in cervical tissue from Suffolk, Belclare, Fur, and NWS only. Cervical tissue was also assessed for the percentage of cervical epithelial populated by mucin secreting goblet cells in the same four ewe breeds. Biochemical analysis showed that there was an effect of ewe breed on sialic acid species, which was represented by Suffolk having higher levels of Neu5,9Ac2 compared with NWS (P |
| Databáze: | OpenAIRE |
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