Estrogen cross-talk with the melatonin signaling pathway in human osteoblasts derived from adolescent idiopathic scoliosis patients
| Autor: | Hubert Labelle, Bouziane Azeddine, Stefan Parent, Florina Moldovan, Kareen Letellier, Alain Moreau, Pierre H. Rompré |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Adolescent medicine.drug_class Blotting Western Gi alpha subunit Fluorescent Antibody Technique Alpha (ethology) GTP-Binding Protein alpha Subunits Gi-Go Biology Melatonin receptor Melatonin Young Adult chemistry.chemical_compound Endocrinology Internal medicine Cyclic AMP GTP-Binding Protein alpha Subunits Gs medicine Humans Immunoprecipitation Cyclic adenosine monophosphate Child Cells Cultured Analysis of Variance Osteoblasts Estradiol Receptor Melatonin MT2 Reverse Transcriptase Polymerase Chain Reaction Receptor Melatonin MT1 Osteoblast medicine.anatomical_structure Scoliosis chemistry Estrogen Case-Control Studies Female Signal transduction hormones hormone substitutes and hormone antagonists Adenylyl Cyclases Signal Transduction medicine.drug |
| Zdroj: | Journal of Pineal Research. 45:383-393 |
| ISSN: | 1600-079X 0742-3098 |
| DOI: | 10.1111/j.1600-079x.2008.00603.x |
| Popis: | Adolescent idiopathic scoliosis (AIS) represents the most frequently occurring form of scoliosis that occurs and progresses in puberty. This critical period coincides with many biological changes related to estrogens. The aim of this study was to determine the effect of 17-beta-estradiol on the responsiveness of AIS osteoblasts to melatonin and the cross-talk between estrogen and melatonin at the levels of the G(S)alpha and G(i)alpha proteins. Human osteoblasts derived from AIS (n = 40) and control patients (n = 10) were first screened for their functional response to the melatonin and 17-beta-estradiol. In response to the 17-beta-estradiol in a specific group of scoliotic patients, the level of 3',5'-cyclic adenosine monophosphate (cAMP) was significantly decreased when compared with the level observed in the presence of increasing concentrations of melatonin alone. Ours results provide strong evidence of the cross-talk between 17-beta-estradiol and melatonin signaling in human AIS osteoblasts. These results indicate a novel role for 17-beta-estradiol and melatonin in AIS, controlling the coupling of G(S)alpha protein and MT2 receptor on human osteoblasts. We found that the increased cAMP levels induced by melatonin can be corrected by the treatment of the cells with 17-beta-estradiol. Thus, estrogens or estrogen receptor agonists become important compounds to consider in AIS osteoblast cell functioning. Consequently, our results add a new facet to the understanding the role and function of melatonin in AIS. |
| Databáze: | OpenAIRE |
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