Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status
| Autor: | Mark A. Febbraio, Tamara L Allen, Emma Estevez, Jessica P. S. Marshall, Mark Read, Darren C. Henstridge, Jereon Zoll, Sarah E Heywood, Helene L. Kammoun, Andrew J. Holmes |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Physiology Endocrinology Diabetes and Metabolism 030209 endocrinology & metabolism White adipose tissue Carbohydrate metabolism Gut flora Diet High-Fat 03 medical and health sciences Mice Random Allocation 0302 clinical medicine Dietary Sucrose Physiology (medical) Internal medicine Physical Conditioning Animal Glucose Intolerance medicine Animals Obesity Feces computer.programming_language Adiposity biology business.industry sed Metabolism Fecal Microbiota Transplantation medicine.disease biology.organism_classification Gastrointestinal Microbiome 030104 developmental biology Endocrinology Glucose Metabolic syndrome Sedentary Behavior business computer |
| Zdroj: | American journal of physiology. Endocrinology and metabolism. 319(1) |
| ISSN: | 1522-1555 |
| Popis: | Studies suggest the gut microbiota contributes to the development of obesity and metabolic syndrome. Exercise alters microbiota composition and diversity and is protective of these maladies. We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets. Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance. Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET). Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed. The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance. No donor group FMT altered recipient body composition. Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient’s microbiota composition observed. FMT can transmit HFHS-induced disrupted glucose metabolism to recipient mice independently of any change in adiposity. However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors. |
| Databáze: | OpenAIRE |
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