The ability of membrane potential dyes and calcafluor white to distinguish between viable and non-viable bacteria
| Autor: | Richard Allman, David Lloyd, R. Lopéz-Amorós, J. M. Stark, David J. Mason |
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| Rok vydání: | 1995 |
| Předmět: |
Salmonella typhimurium
Staphylococcus aureus Colony Count Microbial Biology Bacterial Physiological Phenomena medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Rhodamine 123 Membrane Potentials Flow cytometry Heating chemistry.chemical_compound Escherichia coli medicine Organic Chemicals Fluorescent Dyes Membrane potential Staining and Labeling medicine.diagnostic_test Rhodamines Gramicidin Isoxazoles Carbocyanines Flow Cytometry biology.organism_classification Fluorescence Staining chemistry Biochemistry Barbiturates Bacteria |
| Zdroj: | Journal of Applied Bacteriology. 78:309-315 |
| ISSN: | 0021-8847 |
| DOI: | 10.1111/j.1365-2672.1995.tb05031.x |
| Popis: | Various dyes were assessed for their ability to discriminate between viable and non-viable bacteria. Two methods of killing were employed: by heat treatment or by gramicidin treatment. Staining was carried out in two ways; by staining directly in the medium or by washing cells prior to staining in buffer. Carbocyanine and rhodamine 123 dyes only exhibited small changes in fluorescence between viable and non-viable populations of bacteria. Both oxonol dye (bis 1,3-dibutylbarbituric acid trimethine oxonol) and calcafluor white proved much more useful. |
| Databáze: | OpenAIRE |
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