Use of a Recombinant Antigen (OC 3.6 cDNA) for the Serological Diagnosis of Onchocerciasis in Exposed Nigerian Children
| Autor: | O. O. Kale, R. Chandrashekar, A. F. Ogunrinade, G. J. Weil |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Recombinant antigen
Blotting Western Helminthiasis Nigeria Onchocerciasis Sensitivity and Specificity Serology Western blot Antigen Complementary DNA medicine Animals Humans Serologic Tests Child medicine.diagnostic_test business.industry medicine.disease Recombinant Proteins Infectious Diseases Antigens Helminth Vector (epidemiology) Pediatrics Perinatology and Child Health Immunology Onchocerca business |
| Zdroj: | Journal of Tropical Pediatrics. 38:103-105 |
| ISSN: | 1465-3664 0142-6338 |
| Popis: | We evaluated a recombinant antigen (OC 3.6 cDNA) expressed in pMAL TM vector for the diagnosis of onchocerciasis in children living in an endemic focus of forest-type onchocerciasis in Nigeria. Using the Western blot with the maltose-binding fusion protein as antigen, 91 per cent of mf-positive children and 24 per cent of endemic normal children were positive. Furthermore, age was not a limitation to the assay. The practical limitations of the assay for field diagnosis of onchocerciasis is discussed. |
| Databáze: | OpenAIRE |
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