Preclinical pharmacokinetics of M10 after intragastrical administration of M10-H and M10-Na in Wistar rats
| Autor: | Hui Gan, Ruolan Gu, Taoyun Liu, Jiarui Gao, Zhuona Wu, Suxiang Feng, Xiaoxia Zhu, Guifang Dou, Zhiyun Meng |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Formic acid Electrospray ionization Clinical Biochemistry Anti-Inflammatory Agents Administration Oral Biological Availability Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Limit of Detection Tandem Mass Spectrometry Animals Rats Wistar Chromatography High Pressure Liquid Flavonoids Chromatography 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine Ascorbic acid 0104 chemical sciences Triple quadrupole mass spectrometer Bioavailability Rats chemistry Myricetin Female |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1140 |
| ISSN: | 1873-376X |
| Popis: | As a myricetin derivative, M10 is a potent agent of anti-chronic colonic inflammation. It has better activity than myricetin in preventing azoxymethane/dextran sulfate sodium - induced ulcerative colitis. Here, we introduce a sensitive quantification method based on ultra performance liquid chromatography-tandem mass spectrometry for the determination of M10-H and M10-Na in Wistar rat plasma. Samples were treated with L - ascorbic acid and phosphate buffer solution to maintain stability and with acetonitrile to remove the proteins in the plasma. The supernatant was separated with BEH C18 column and eluted with ultrapure water and acetonitrile both containing 0.1% formic acid. The detection was performed by a triple quadrupole mass spectrometer with positive electrospray ionization mode in multiple reactive monitoring. This method was validated for the carryover effect, selectivity, accuracy, precision, matrix effect, stability, and recovery. A linear correlation was established between concentration and response by the calibration curves over 10–2000 ng·mL−1 (r > 0.99). This method was applied to a pharmacokinetic study of intragastrical administration of M10-H and M10-Na in Wistar rats. In addition, the relative bioavailability of M10-H to M10-Na in Wistar rats was 60 ± 19%, calculated by the ratio of area under concentration (AUC) of M10-H to M10-Na after intragastrical administration of a single dose (100 mg·kg−1 for M10-H and M10-Na, respectively) in Wistar rats. |
| Databáze: | OpenAIRE |
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