Ribosome Stalling Regulates IRES-Mediated Translation in Eukaryotes, a Parallel to Prokaryotic Attenuation
| Autor: | Anton A. Komar, Randal J. Kaufman, James Fernandez, Martin D. Snider, Ibrahim Yaman, Mark G. Caprara, Haiyan Liu, Alex B. Lopez, William C. Merrick, Charles Huang, Wouter H. Lamers, Maria Hatzoglou |
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| Přispěvatelé: | AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Medical Biology, Anatomie en Embryologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism |
| Rok vydání: | 2005 |
| Předmět: |
Five prime untranslated region
Eukaryotic Initiation Factor-2 Molecular Sequence Data Codon Initiator In Vitro Techniques Biology Transfection Models Biological Cell Line Mice Open Reading Frames 03 medical and health sciences Eukaryotic translation Prokaryotic translation Animals Amino Acid Sequence RNA Messenger Cycloheximide Phosphorylation Molecular Biology Cationic Amino Acid Transporter 1 030304 developmental biology 0303 health sciences Base Sequence 030302 biochemistry & molecular biology EIF4E fungi Translation (biology) Cell Biology Molecular biology Rats Ribosomal binding site Cell biology Internal ribosome entry site Eukaryotic Cells Prokaryotic Cells Protein Biosynthesis Codon Terminator Nucleic Acid Conformation Rabbits Ribosomes EF-Tu |
| Zdroj: | Molecular cell, 17(3), 405-416. Cell Press Molecular Cell, 17(3), 405-416. Cell Press |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2004.12.024 |
| Popis: | It was previously shown that the mRNA for the cat-1 Arg/Lys transporter is translated from an internal ribosome entry site (IRES) that is regulated by cellular stress. Amino acid starvation stimulated cat-1 translation via a mechanism that requires translation of an ORF in the mRNA leader and remodeling of the leader to form an active IRES (the "zipper model" of translational control). It is shown here that slowing of the leader peptide elongation rate, either by cycloheximide or the introduction of rare codons, stimulated translation of the downstream ORF. These results suggest that ribosome stalling in the upstream ORF causes mRNA remodeling and formation of an active IRES. This control is reminiscent of translation attenuation in prokaryotic operons, where inhibition of translation elongation can regulate both mRNA translation and gene transcription by altering mRNA structure. |
| Databáze: | OpenAIRE |
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