Expression of orexin B and its receptor 2 in rat testis
| Autor: | Alfredo Vittoria, Nicola Mirabella, Luigi Michele Pavone, E. Langella, Simona Tafuri, Giovanna Liguori, Loredana Assisi, Anna Costagliola |
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| Přispěvatelé: | Liguori, Giovanna, Pavone, LUIGI MICHELE, Assisi, Loredana, Langella, Emilia, Tafuri, Simona, Mirabella, Nicola, Costagliola, Anna, Vittoria, Alfredo |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Receptors Neuropeptide medicine.medical_specialty Hypothalamus Neuropeptide Biology Receptors G-Protein-Coupled 03 medical and health sciences Orexin-A Endocrinology Orexin 2 receptor Orexin Receptors Internal medicine Testis medicine Animals Orexin B Receptor Messenger RNA Orexins Medicine (all) Neuropeptides Intracellular Signaling Peptides and Proteins Spermatids Orexin receptor Epithelium Orexin Cell biology Rats Blot 030104 developmental biology medicine.anatomical_structure Testi Rat Steroidogenesi Animal Science and Zoology |
| Zdroj: | General and comparative endocrinology. 242 |
| ISSN: | 1095-6840 |
| Popis: | The peptides orexin A (OxA) and orexin B (OxB) deriving from a common precursor molecule, prepro-orexin, by proteolytic cleavage, bind the two G-coupled OX1 and OX2 receptors. While OX1 selectively binds OxA, OX2 shows similar affinity for both orexins. Firstly discovered in the hypothalamus, orexins and their receptors have been found in other brain regions as well as in peripheral tissues of mammals, thus resulting involved in the regulation of a broad variety of physiological functions. While the functional localization of OxA and OX1 in the mammalian genital tract has been already described, the expression of OxB and OX2 and their potential role in the reproductive functions remain to be explored. Here, we investigated the presence of OxB and OX2 in the rat testis by immunohistochemical and biochemical analyses. The results definitely demonstrated the localization of OxB and OX2 in pachytene and second spermatocytes as well as in spermatids at all stages of the cycle of the seminiferous epithelium. The expression of both OX2 mRNA and protein in the rat testis was also established by RT-PCR and Western blotting, respectively. The analysis of the molecular mechanism of action of OxB in the rat testis showed that OxB, in contrast with OxA, is unable to promote steroidogenesis. These results translate into the regulation of diverse biological actions by OxA and OxB in the male gonad. |
| Databáze: | OpenAIRE |
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