Catesbeianin-1, a novel antimicrobial peptide isolated from the skin of Lithobates catesbeianus (American bullfrog)
| Autor: | Wenyu Han, Huihui Xu, Kunyuan Tie, Xin Feng, Yuan Cao, Yang Zhang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Erythrocytes Cell Survival Bioengineering Peptide Microbial Sensitivity Tests medicine.disease_cause Applied Microbiology and Biotechnology Hemolysis Microbiology 03 medical and health sciences Mice Pepsin Anti-Infective Agents Bullfrog medicine Animals Humans Skin chemistry.chemical_classification Mice Inbred BALB C Rana catesbeiana 030102 biochemistry & molecular biology biology Bacteria Lithobates General Medicine Antimicrobial biology.organism_classification Trypsin 030104 developmental biology HEK293 Cells chemistry Staphylococcus aureus biology.protein Female Biotechnology medicine.drug Antimicrobial Cationic Peptides |
| Zdroj: | Biotechnology letters. 39(6) |
| ISSN: | 1573-6776 |
| Popis: | To identify and characterize a novel antimicrobial peptide, catesbeianin-1. Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 exhibited moderate hemolytic activity (approx 8%) at 100 μg/ml, and its HC50 (50% hemolytic concentration) was 300 μg/ml. Its cytotoxicity was approx 10–20% at 100 μg/ml, and its CC50 (50% cytotoxic concentration) was >100 μg/ml. The LD50 of catesbeianin-1 in mice was 80 mg/kg. At 3.1 µg/ml, catesbeianin-1 significantly inhibited the growth of methicillin-resistant Staphylococcus aureus. A new antimicrobial peptide from the skin of Lithobates catesbeianus (American bullfrog) may represent a template for the development of novel antimicrobial agents. |
| Databáze: | OpenAIRE |
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