Comparison of the Effect of Skin Preparation Pads on Transepidermal Water Loss in Ex Vivo Human Skin
| Autor: | Jean-Michel Linget, Orla Duffy-Roger, Magali Kouidhi, Claire E. Wilson, Hanan Osman-Ponchet, Jeanne Marceau-Suissa, Alexandre Gaborit, Sandrine Anglars |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Tape stripping Transepidermal water loss Human skin Dermatology Andrology 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Methyl aminolevulinate medicine Stratum corneum Barrier function Original Research Skin preparation pad integumentary system business.industry Ex vivo human skin Plastic surgery medicine.anatomical_structure 030220 oncology & carcinogenesis Epidermis business Ex vivo medicine.drug |
| Zdroj: | Dermatology and Therapy |
| ISSN: | 2193-8210 |
| Popis: | Introduction Pre-treatment of the skin to remove scales and crusts prior to photodynamic therapy (PDT) is essential to enhance the uptake of topically applied methyl aminolevulinate (MAL) and to improve treatment efficacy. This study compared the effect of two different skin preparation pads on skin integrity in ex vivo human skin. Methods Ex vivo human skin samples from three donors were pre-treated in triplicates with PREPSTER™ (PR) skin preparation pad (6, 8, and 10 passages) or Ambu Unilect™ (A-UN) skin preparation pad (6, 8, and 10 passages). In addition, skin samples were pre-treated with tape strippings (10 adhesive tape strips) as a reference method for comparison. Transepidermal water loss (TEWL) was measured on intact skin and following skin barrier impairment using skin preparation pads and tape stripping. Histological analysis was performed to verify the impairment of the stratum corneum (SC) barrier function in samples from intact skin (control), 10 tape strippings (reference method), 10 passages of PR, and 10 passages of A-UN. Results TEWL increased with the increasing number of passages of skin preparation pads, with 2.4- and 3.3-fold increases following 10 passages of A-UN and PR, respectively, versus a 2.2-fold increase with 10 tape strippings (reference). Histological analysis showed only partial removal of the SC, with no damage observed on the epidermis, regardless of the procedure used. Conclusion Pre-treatment of skin using PR and A-UN skin preparation pads markedly increases TEWL, indicating slight impairment of the SC barrier function. Comparison of both skin preparation pads showed that PR pad consistently induced significantly higher TEWL than A-UN pad (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|