Evaluating the accuracy and diagnostic value of CFW and a new fluorescent reagents, fluorescent brightener 85, for the diagnosis of vulvovaginal candidiasis

Autor: Xueping Yue, Zixuan Yu, Yunzhuan Zhao
Rok vydání: 2021
Předmět:
Adult
Microbiology (medical)
medicine.medical_specialty
Time Factors
Diagnostic methods
Potassium Compounds
Clinical Biochemistry
Candida glabrata
Sensitivity and Specificity
Gastroenterology
CFW
Smear microscopy
McNemar's test
Internal medicine
Candida albicans
parasitic diseases
Hydroxides
Image Processing
Computer-Assisted
medicine
Humans
Immunology and Allergy
Candida tropicalis
Vaginal secretion
Research Articles
fluorescent method
Candidiasis
Vulvovaginal
Fluorescent Dyes
Vaginitis
vulvovaginal candidiasis (VVC)
business.industry
Benzenesulfonates
Biochemistry (medical)
Significant difference
Public Health
Environmental and Occupational Health
Hematology
medicine.disease
Fluorescence
potassium hydroxide (KOH) smear microscopy
FB85
Medical Laboratory Technology
Microscopy
Fluorescence
Vulvovaginal Candidiasis
Female
business
Research Article
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
0887-8013
DOI: 10.1002/jcla.23891
Popis: Background Vulvovaginal candidiasis (VVC) is a common vaginitis in females. The commonly used diagnostic method, 10% potassium hydroxide (KOH) smear microscopy, makes it not very easy to recognize fungi. Methods Vaginal secretions were collected from clinically suspected VVC patients and divided into four groups and examined using KOH, CFW (Calcofluor White), FB 85(fluorescent brightener 85), and culture. The data were statistically analyzed. Results In total, 110 patients with suspected VVC were recruited. The positive rates of KOH, CFW, FB 85, and the culture method were 68.2%, 64.5%, 61.8%, and 77%, respectively. According to the McNemar test, there was no statistically significant difference between the KOH, CFW, and the FB 85 methods (p > 0.05). However, CFW had a shorter diagnosis time than the KOH method and had a statistically significant difference (p
The images under fluorescence microscopy and light microscopy with four methods. a‐d) show C. albicans, and e‐h) show C. glabrata. Both the fungal color and the structure in FB 85 and CFW were clear and easily recognized from the background squamous cells compared with the KOH method. a) The KOH method with C. albicans under light microscopy. b) The CFW method with C. albicans under fluorescence microscopy. c) The FB 85 method with C. albicans under fluorescence microscopy. d) The culture method with C. albicans under fluorescence microscopy with CFW. e) The KOH method with C. glabrata under light microscopy. f) The CFW method with C. glabrata under fluorescence microscopy. g) The FB 85 method with C. glabrata under fluorescence microscopy. h) The culture method with C. glabrata under fluorescence microscopy with CFW.
Databáze: OpenAIRE
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