Abnormal increase of miR-4262 promotes cell proliferation and migration by targeting large tumor suppressor 1 in gliomas
| Autor: | Tianwei Jiang, Suinuan Wang, Yitao Qian, Tao Ma, Changchun Yang, Ya Peng, Chunbo Liu, Geng Jia, Rong Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine MMP2 Down-Regulation Protein Serine-Threonine Kinases Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Cell Line Tumor Glioma Matrix Metalloproteinase 13 microRNA medicine Humans Aged Cell Proliferation Migration Assay Cell growth Chemistry Computational Biology Cancer Cell Biology Middle Aged medicine.disease Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer cell Disease Progression Cancer research Matrix Metalloproteinase 2 Female |
| Zdroj: | Pathology - Research and Practice. 216:152778 |
| ISSN: | 0344-0338 |
| DOI: | 10.1016/j.prp.2019.152778 |
| Popis: | Background miRNA was recently detected as tumor suppressor or inducer in various cancers including gliomas. Due to the abnormal expression of miR-4262 in glioma cancer, we supposed that miR-4262 made efforts in proliferation and migration in glioma cancer. Methods CCK-8, Transwell migration Assay and Wound-healing assay were appraisal assays for cell proliferation and migration. qRT-PCR and western blot were performed to test the expression of miR-4262, MMP2, MMP13 and LATS1 in glioma cancers tissues and cancer cells. The targeting detection between miR-4262 and LATS1 was detected by luciferase reporter assay. Results miR-4262 expression was dramatically higher in glioma tumor tissues than in para-tumor control. Inhibition of miR-4262 in glioma cancer cells prominently inhibited cell proliferation and migration. Mechanically, downregulation of miR-4262 inhibited expression of matrix metalloproteinase (MMP) -2, -13. In addition, miR-4262 directly and negatively modulated expression of large tumor suppressor 1 (LATS1). Moreover, we discovered that overexpression of LATS1 could reverse the effects of miR-4262 on cell proliferation and migration, as well as the production of MMP-2, -13. Conclusions In glioma cancer, miR-4262 regulated cell proliferation and migration mediated by LATS1. This indicated that miR-4262 is a tumor inducer in glioma cancer and may be a feasible target for glioma therapy. |
| Databáze: | OpenAIRE |
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