Activity‐Based Protein Profiling of Chitin Catabolism
| Autor: | Isaac K. Attah, Elias K. Zegeye, Christopher R. Anderton, Natalie C. Sadler, Janet K. Jansson, Gerard X. Lomas, Aaron T. Wright, Kirsten S. Hofmockel |
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| Rok vydání: | 2020 |
| Předmět: |
Proteome
Chitin Biochemistry Cellvibrio chemistry.chemical_compound Bacterial Proteins Glucosamine Molecular Biology Cellvibrio japonicus chemistry.chemical_classification biology Catabolism Hydrolysis Chitinases fungi Organic Chemistry Activity-based proteomics biology.organism_classification Enzyme chemistry Chitinase biology.protein Molecular Medicine Function (biology) |
| Zdroj: | ChemBioChem. 22:717-723 |
| ISSN: | 1439-7633 1439-4227 |
| DOI: | 10.1002/cbic.202000616 |
| Popis: | The microbial catabolism of chitin, an abundant and ubiquitous environmental organic polymer, is a fundamental cog in terrestrial and aquatic carbon and nitrogen cycles. Despite the importance of this critical bio-geochemical function, there is a limited understanding of the synergy between the various hydrolytic and accessory enzymes involved in chitin catabolism. To address this deficit, we synthesized activity-based probes (ABPs) designed to target active chitinolytic enzymes by modifying the chitin subunits N-acetyl glucosamine and chitotriose. The ABPs were used to determine the active complement of chitinolytic enzymes produced over time by the soil bacterium Cellvibrio japonicus treated with various C substrates. We demonstrate the utility of these ABPs in determining the synergy between various enzymes involved in chitin catabolism. The strategy can be used to gain molecular-level insights that can be used to better understand microbial roles in soil bio-geochemical cycling in the face of a changing climate. |
| Databáze: | OpenAIRE |
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