Platelet Induced Functional Alteration of CD4+ and CD8+ T Cells in HNSCC
| Autor: | Christina Polasky, Ralph Pries, Franziska Wendt, Barbara Wollenberg |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type medicine.medical_treatment T cell T cells Peripheral blood mononuclear cell PD-1/PD-L1 HNSCC Catalysis Flow cytometry lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Immune system platelets immune regulation medicine Cytotoxic T cell Platelet Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy medicine.diagnostic_test Chemistry Organic Chemistry General Medicine Computer Science Applications Cell biology ddc 030104 developmental biology Cytokine medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 030220 oncology & carcinogenesis |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 20 International Journal of Molecular Sciences, Vol 21, Iss 7507, p 7507 (2020) |
| Popis: | Platelets (PLT) are the second most abundant cell type in human blood and exert various immune-regulatory functions under both physiological and pathological conditions. In fact, immune cell regulation via platelets has been demonstrated in several studies within the past decade. However, the exact mechanisms behind T cell regulation remain poorly understood. We questioned whether the formation of aggregates of platelets and T cells has an impact on T-cell functions. In the present study, we stimulated PBMC cultures with anti-CD3 and anti-CD28 mABs and cultured them at a PLT: PBMC ratio of 1:1 or 100:1. After 24, 48, and 72 h, PD-1, PD-L1 expression, and proliferation were analyzed on T cells using flow cytometry. Cytokine production was measured in PHA stimulated CD4 cells after 6 h. We found a significant platelet-mediated decrease in PD-1 and PD-L1 expression, proliferation, as well as IFN-&gamma and TNF-&alpha production. Perturbations also at least partially remained after spatial separation of PLTs from PBMCs in Transwell-assays. T cell-platelet aggregates showed similar levels of activation markers, proliferation, and secreted cytokines as their non-complexed counterparts. Results indicate a platelet mediated regulation of T cells via direct and indirect contact, but only mediocre effects of the complex formation itself. |
| Databáze: | OpenAIRE |
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