The nature of membrane anchorage determines kinase association and detergent solubility of CD4
| Autor: | Mark A. Mallory, Katherine Hanify, Robert W. Finberg, Keith R. Solomon |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Vesicle-associated membrane protein 8
Glycosylphosphatidylinositols Octoxynol Detergents Biophysics Biology Transfection Biochemistry Iodoacetamide Glucosides Tumor Cells Cultured Humans Solubility Molecular Biology Integral membrane protein Kinase Peripheral membrane protein Membrane Proteins Cell Biology Phosphoproteins Precipitin Tests carbohydrates (lipids) Membrane Membrane protein Gene Expression Regulation Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Phosphoprotein CD4 Antigens lipids (amino acids peptides and proteins) Electrophoresis Polyacrylamide Gel Protein Kinases |
| Zdroj: | Biochemical and biophysical research communications. 242(2) |
| ISSN: | 0006-291X |
| Popis: | The presence of a glycosylphosphatidylinositol (GPI) anchor on a membrane protein is thought to influence aspects of the protein's biochemistry. While it has been demonstrated that a GPI-anchor is sufficient for altering the detergent solubility of integral membrane proteins, it has not been shown that the anchor is sufficient for changing the phosphoprotein associations of membrane proteins. In order to define the influence of GPI-anchors on the biochemistry of membrane proteins we compared the phosphoprotein associations and detergent solubility of wild-type and GPI-anchored CD4 expressed on HSB cell transfectants. While wild-type CD4 was mostly associated with lck kinase, GPI-anchored CD4 was associated with the 'GPI-anchored pattern of phosphoproteins'. The Triton X-100 solubilities of the two forms of CD4 were also distinct: wild-type CD4 was > 95% soluble, whereas GPI-anchored CD4 was only 65% soluble. These results underscore the deterministic role of the GPI-anchor in the properties associated with GPI-anchored proteins. |
| Databáze: | OpenAIRE |
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