Bis(2-sulfanylethyl)amino native peptide ligation
| Autor: | Annick Blanpain, Nathalie Ollivier, Julien Dheur, Reda Mhidia, Oleg Melnyk |
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| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Protein chemical synthesis Molecular Structure Organic Chemistry Regioselectivity Peptide Stereoisomerism Biochemistry Combinatorial chemistry chemistry.chemical_compound chemistry Peptide synthesis Peptide bond Chemical ligation Sulfhydryl Compounds Physical and Theoretical Chemistry Ligation Peptides |
| Zdroj: | Organic letters. 12(22) |
| ISSN: | 1523-7052 |
| Popis: | The reaction of a peptide featuring a bis(2-sulfanylethyl)amino (SEA) group on its C-terminus with a cysteinyl peptide in water at pH 7 and 37 °C leads to the chemoselective and regioselective formation of a native peptide bond. This method called SEA ligation enriches the native peptide ligation repertoire available to the peptide chemist. Preparation of an innovative solid support which allows the straightforward synthesis of peptide SEA fragments using standard Fmoc/tert-butyl solid phase peptide synthesis procedures is also described. |
| Databáze: | OpenAIRE |
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