Substitution mapping of a blood pressure quantitative trait locus to a 2.73 Mb region on rat chromosome 1
| Autor: | John P. Rapp, Michael R. Garrett, Howard Dene, Bina Joe |
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| Rok vydání: | 2003 |
| Předmět: |
Genetics
Candidate gene Genome Rats Inbred Dahl Physiology Quantitative Trait Loci Congenic Chromosome Mapping Sequence assembly Blood Pressure Locus (genetics) Biology Quantitative trait locus Rats Centimorgan Blood pressure Animals Congenic Rats Inbred Lew Internal Medicine Animals Cardiology and Cardiovascular Medicine Gene |
| Zdroj: | Journal of Hypertension. 21:2077-2084 |
| ISSN: | 0263-6352 |
| Popis: | OBJECTIVE To improve the localization of a blood pressure quantitative trait locus (BP QTL) on rat chromosome (RNO) 1. METHODS Congenic substrains were derived from the progenitor congenic strains S.LEW(D1Mco4X1) and S.LEW(D1Mco4X5) which previously localized a BP QTL (region 2) to a 17cM interval on RNO1. The newly developed congenic substrains, along with control Dahl salt-sensitive (S) rats were fed a 2% NaCl diet for 24 days before their BP was compared by both tail-cuff and radiotelemetry methods. RESULTS By comparing BP of these congenic substrains to that of S rats, we have refined the location of the BP QTL2 region to a 2.73 Mb genomic interval that contains 19 annotated genes in the latest rat genome assembly (version 2.1). Slc9a3, the gene encoding the Na(+)/H(+) exchanger 3, originally a candidate gene in the BP QTL2 region, is excluded based on its map location. CONCLUSION Substitution mapping was used to reduce a BP QTL on RNO1 from 17 centimorgans (cM) to approximately 1.4 cM (= 2.73 Mb). This region now contains 19 annotated rat candidate genes. |
| Databáze: | OpenAIRE |
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