Apoptosis in rabbit embryos produced by fertilization or nuclear transfer with fibroblasts and cumulus cells
| Autor: | Zi-Li Lei, Yue-Liang Zheng, Li-Sheng Zhang, Qing-Yuan Sun, Shu-Zhen Liu, Da-Yuan Chen, Yan-Ling Zhang, Xiang-Fen Song, Li-Juan Yao, Man-Xi Jiang |
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| Rok vydání: | 2005 |
| Předmět: |
Embryology
Programmed cell death Nuclear Transfer Techniques Cleavage Stage Ovum Cloning Organism Embryonic Development Apoptosis Fertilization in Vitro Biology Andrology Endocrinology Pregnancy In Situ Nick-End Labeling medicine Inner cell mass Animals Blastocyst reproductive and urinary physiology TUNEL assay Microscopy Confocal urogenital system Embryogenesis Obstetrics and Gynecology Embryo Cell Biology Fibroblasts Embryo Mammalian Embryonic stem cell medicine.anatomical_structure Reproductive Medicine embryonic structures Immunology Female Rabbits |
| Zdroj: | Reproduction (Cambridge, England). 130(3) |
| ISSN: | 1470-1626 |
| Popis: | In this study, we investigated the development, the cell number of the blastocyst, and apoptosis in rabbit nuclear transfer (NT) embryos derived from adult fibroblasts and cumulus cells as compared with embryos derived fromin vivofertilization andin vitroculture. The developmental rate and the total cell number of the blastocyst were significantly lower in NT embryos than in fertilized embryos (FEs). The type of donor cells did not affect the embryonic developmental rate and the total cell number of blastocysts in NT groups. The present study investigated the onset and the frequency of apoptosis in NT embryos and FEs by using a terminal deoxynucleotidyl transferase-mediated dUTP nick and labeling (TUNEL) assay. The earliest positive TUNEL signals were detected at the eight-cell stage in NT embryos and at the morula stage in FEs. The apoptotic index of the total blastocysts, the inner cell mass and the trophoderm was greatly higher in the NT embryos than in FEs. Moreover, the apoptotic index of the blastocyst from fibroblasts was significantly higher than that of the blastocyst from cumulus cells. |
| Databáze: | OpenAIRE |
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