Nonspecific genetic regulation of antibody responsiveness in the mouse
Autor: | G. Biozzi, M. Siqueira, Vera C. A. Ferreira, Olga M. Ibañez, Osvaldo A. Sant'Anna, M B Esteves, Reis Mh |
---|---|
Rok vydání: | 1977 |
Předmět: |
Salmonella typhimurium
Erythrocytes Somatic cell Immunology chemical and pharmacologic phenomena Cross Reactions Selective breeding Epitopes Mice Agglutinin Antigen Salmonella Antigens Heterophile Immunology and Allergy Animals Antigens Antigens Bacterial biology Hemagglutination Tests Molecular biology Phenotype Antibodies Bacterial Dinitrobenzenes Antibody response Genes Flagella Immunoglobulin G Immune response genes Antibody Formation biology.protein Antibody |
Zdroj: | European journal of immunology. 7(4) |
ISSN: | 0014-2980 |
Popis: | Four lines of mice were produced by selective breeding for quantitative agglutinin responsiveness to flagellar (f) or somatic (s) antigens (Ags) of Salmonellae: high (H) or low (L) responder lines to f Ag and H and L responder lines to sAg. The Salmonellae contained both f and sAgs, the Ag used to perform the selection was the Selection Ag and the other was the Associated Ag. The selective breeding produced a progressive interline separation with an equivalent effect for both Ags. After 15 generations (F15) the level of agglutinin response was about 60 times higher in H than in L responders. About 50 % of the phenotypic variation of the character investigated is determined by a group of immune response genes, the rest is due to environmental factors. The nonspecific effect of this group of immune response genes was investigated by measuring the responses to three independent antigens: Sheep erythrocytes (SE), dinitrophenyl-conjugated human IgG (DNP-HGG) and bovine IgG (BGG). The selection for fAg response produced an equivalent modification in the responsiveness to the Associated Ag (97 %) and to BGG (130 %). This nonspecific effect was smaller for responsiveness to SE and DNP-HGG, 58 % and 41 % of the Selection Ag response, respectively. The selection for sAg response produced a nonspecific modification of responsiveness of 94 % for the Associated Ag of 74 % for BGG and 63 % for DNP-HGG. An important exception concerned SE to which an equal antibody response is produced in high and low lines of sAg selection. |
Databáze: | OpenAIRE |
Externí odkaz: |