Perinatal thymocyte antigen expression and postnatal immune development altered by gestational exposure to tetrachlorodibenzo-p-dioxin (TCDD)
| Autor: | C E Comment, Dori R. Germolec, J. J. Heindell, P. Lindstrom, Michael I. Luster, B L Blaylock, S. D. Holladay |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cytotoxicity
Immunologic Male Embryology medicine.medical_specialty Polychlorinated Dibenzodioxins Offspring CD8 Antigens T-Lymphocytes Health Toxicology and Mutagenesis Population CD4-CD8 Ratio Thymus Gland Toxicology Mice Immune system Antigen Pregnancy Internal medicine medicine Animals Cytotoxic T cell education Maternal-Fetal Exchange Fetus education.field_of_study biology Flow Cytometry Mice Inbred C57BL Thymocyte Phenotype Endocrinology Antigens Surface CD4 Antigens biology.protein Thy-1 Antigens Female Antibody T-Lymphocytes Cytotoxic Developmental Biology |
| Zdroj: | Teratology. 44:385-393 |
| ISSN: | 1096-9926 0040-3709 |
| DOI: | 10.1002/tera.1420440405 |
| Popis: | In utero exposure to the environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was found to alter expression of murine thymocyte fetal cell-surface markers. Pregnant mice were treated (via gavage) with 0, 1.5, or 3.0 micrograms TCDD/kg/day in corn oil on gestational days (gd) 6-14. Offspring were examined on gd 18 and postnatally on d6, d14, and d21, and at 7, 8, and 10 weeks of age. Severe thymic atrophy and cellular depletion were found both pre- and postnatally in TCDD-exposed mice. Immunocytochemical localization of the Thy 1.2 antigen on gd 18 thymocytes revealed no TCDD-related changes in cellular distribution. Flow cytometric analysis, however, indicated that the TCDD treatment resulted in a significant decrease in the percentage of CD4+8+ fetal thymocytes, as well as significantly increased CD4-8- and CD4-8+ thymocytes. The increased CD4-8+ population after TCDD was not from induction of Ts cells. At 7-8 weeks postnatally, no differences existed between control and treatment groups in mitogen responses and antibody plaque response. However, altered thymocyte antigen expression was found to correlate with altered postnatal immune function, as evidenced by decreased cytotoxic T lymphocyte response at 8 weeks of age. Taken together, these results indicate that immunosuppression following prenatal exposure to TCDD can be readily detected by qualitative and quantitative changes in the cell surface phenotype of fetal thymocytes. Furthermore, the observed altered distribution suggests that TCDD inhibits normal thymocyte maturational processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text