The program of androgen-responsive genes in neoplastic prostate epithelium
| Autor: | Camari Ferguson, Nigel J. Clegg, Hugh Arnold, Leroy Hood, Michael Bonham, Peter S. Nelson, James F. White, Biaoyang Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.drug_class Cellular differentiation Amino Acid Motifs Molecular Sequence Data Biology Gene expression LNCaP medicine Tumor Cells Cultured Humans Amino Acid Sequence Gene Genetics Multidisciplinary Sequence Homology Amino Acid Prostatic Neoplasms Biological Transport Cell Differentiation Biological Sciences Androgen Androgen receptor Gene Expression Regulation Neoplastic Dihydrotestosterone Androgens Human genome Cell Division medicine.drug |
| Popis: | The human prostate gland is an important target organ of androgenic hormones. Testosterone and dihydrotestosterone interact with the androgen receptor to regulate vital aspects of prostate growth and function including cellular proliferation, differentiation, apoptosis, metabolism, and secretory activity. Our objective in this study was to characterize the temporal program of transcription that reflects the cellular response to androgens and to identify specific androgen-regulated genes (ARGs) or gene networks that participate in these responses. We used cDNA microarrays representing about 20,000 distinct human genes to profile androgen-responsive transcripts in the LNCaP adenocarcinoma cell line and identified 146 genes with transcript alterations more than 3-fold. Of these, 103 encode proteins with described functional roles, and 43 represent transcripts that have yet to be characterized. Temporal gene expression profiles grouped the ARGs into four distinct cohorts. Five uncharacterized ARGs demonstrated exclusive or high expression levels in the prostate relative to other tissues studied. A search of available DNA sequence upstream of 28 ARGs identified 25 with homology to the androgen response-element consensus-binding motif. These results identify previously uncharacterized and unsuspected genes whose expression levels are directly or indirectly regulated by androgens; further, they provide a comprehensive temporal view of the transcriptional program of human androgen-responsive cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|