Propionibacterium namnetense sp. nov., isolated from a human bone infection
| Autor: | Guillaume Ghislain Aubin, Pascale Bémer, Stéphane Corvec, Olivier Lemenand, Stanimir Kambarev, Jocelyne Caillon, Paul A. Lawson, Nisha B. Patel |
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| Přispěvatelé: | Bernardo, Elizabeth, Département de Bactériologie et d'Hygiène [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), Thérapeutiques cliniques et expérimentales des infections (EA 3826) (EA 3826), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Nuclear Oncology (CRCINA-ÉQUIPE 13), Centre de Recherche en Cancérologie et Immunologie Nantes-Angers (CRCINA), Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université d'Angers (UA)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre hospitalier universitaire de Nantes (CHU Nantes), Department of Microbiology and Plant Biology [Norman, Oklahoma, USA], University of Oklahoma (OU), Laboratoire de microbiologie [CH Saint Nazaire], Centre hospitalier de Saint-Nazaire, Clinical and Translational Research in Skin Cancer (CRCINA-ÉQUIPE 2), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Centre National de la Recherche Scientifique (CNRS)-Université d'Angers (UA) |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial Male 0301 basic medicine 030106 microbiology [SDV.CAN]Life Sciences [q-bio]/Cancer medicine.disease_cause Microbiology Genome Young Adult 03 medical and health sciences Propionibacterium acnes [SDV.CAN] Life Sciences [q-bio]/Cancer RNA Ribosomal 16S medicine Humans Gene Phylogeny Ecology Evolution Behavior and Systematics Base Composition Strain (chemistry) Phylogenetic tree biology Fatty Acids Propionibacterium Nucleic Acid Hybridization Sequence Analysis DNA General Medicine Bone Diseases Infectious 16S ribosomal RNA biology.organism_classification rpoB [Propionibacterium] namnetense Bacterial Typing Techniques France |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, 2016, 66 (9), pp.3393-3399. ⟨10.1099/ijsem.0.001204⟩ International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2016, 66 (9), pp.3393-3399. ⟨10.1099/ijsem.0.001204⟩ |
| ISSN: | 1466-5034 1466-5026 3130-7302 |
| Popis: | International audience; A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus Propionibacterium, most closely related to Propionibacterium acnes subsp. acnes and Propionibacterium acnes subsp. elongatum with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for rpoB, recA and gyrB clearly separated the novel organism from all species and subspecies of the genus Propionibacterium. However, a DNA–DNA hybridization analysis between the novel organism and the type strain P. acnes ATCC 6919[T] revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cutoff value of 95 % for species delineation. The major fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 and iso-C 17 : 0. The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of P. acnes On the basis of the results presented, the organism represents a novel member of the genus Propionibacterium for which the name Propionibacterium namnetense sp. nov. is proposed. The type strain is NTS 31307302[T] (=DSM 29427[T] =CCUG 66358[T]). |
| Databáze: | OpenAIRE |
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