Preconceptional allergen immunization can induce offspring IL-17 secreting B cells (B17): do they share similarities with regulatory B10 cells?
| Autor: | Giovanna Rossi Beltrame, Aline Aparecida de Lima Lira, Amanda Harumi Sabô Inoue, Jefferson Russo Victor, Alberto José da Silva Duarte, Marília Garcia de-Oliveira |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Pulmonary and Respiratory Medicine Ovalbumin Offspring Primary Cell Culture Immunology B-Lymphocyte Subsets chemical and pharmacologic phenomena medicine.disease_cause ALBUMINAS Andrology Mice 03 medical and health sciences Neonatal immunization 0302 clinical medicine Allergen Hypersensitivity Animals Immunology and Allergy Medicine Cells Cultured Mice Knockout business.industry Interleukin-17 General Medicine Allergens biochemical phenomena metabolism and nutrition Interleukin-10 Mice Inbred C57BL Fluorescence intensity 030104 developmental biology Immunization Models Animal Female Interleukin 17 business Immunity Maternally-Acquired 030215 immunology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0301-0546 |
| DOI: | 10.1016/j.aller.2018.04.001 |
| Popis: | Background IL-17-producing B cells can be identified in both mice and human and were named B17 cells. The role of B17 cells still needs to be elucidated and its inflammatory or regulatory functions remain controversial. Objective We evaluate the effect of maternal immunization with OVA on offspring B cells that produces IL-17 and can show a regulatory potential by IL-10 production. Methods C57BL/6 WT, IL-10−/− or CD28−/− female mice were immunized or not with OVA in Alum, and immunized females were boosted after 10 and 20 days. Immunized and non-immunized females were mated, and pups from both groups were evaluated at 3 or 20 days old (d.o.). Some offspring from the aforementioned two groups were immunized with OVA at 3 d.o., boosted after 10 days and evaluated at 20 d.o. Results Maternal immunization with OVA induced offspring B cells to produce IL-17 at higher intensity compared to the control group of offspring at 3 d.o. This effect was maintained until 20 d.o. and even after neonatal immunization with OVA. The co-production of IL-10 on offspring IL-17 + B cells is up-regulated in response to maternal immunization with OVA. Maternal immunization with OVA on IL-10−/− mice reveals reduced percentage and mean of fluorescence intensity of IL-17 on B cells of offspring. Conclusion Preconception OVA immunization can induce offspring B cells that produce IL-17 at higher intensity and co-produce mainly IL-10. This could be the reason why B17 cells had been described in the literature with controversial roles upon their regulatory function. |
| Databáze: | OpenAIRE |
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