Large-Scale Isolation of Human Hepatocytes for Therapeutic Application
| Autor: | Martí Manyalich I Vidal, Marc Barthold, Hans-Gert Heuft, Carsten Griesel, Michael Winkler, Marc Net, Lubomir Arseniev, Harald Schrem, Timm Bredehorn, Michael P. Manns, Michael Ott, Krassimira Alexandrova, Sabine Kafert-Kasting |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
0301 basic medicine
Adult Male Quality Control Tissue and Organ Procurement Isolation (health care) Cell Survival Cell Transplantation Biomedical Engineering lcsh:Medicine Cell Count Guidelines as Topic Cell Separation Bioinformatics 03 medical and health sciences 0302 clinical medicine Cell transplantation Hepatocyte transplantation Chronic liver failure Medicine Humans Cell survival Cells Cultured Aged Cryopreservation Transplantation business.industry lcsh:R Cell Biology Middle Aged Tissue Donors Fatty Liver 030104 developmental biology Hepatocytes Female business 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 14 (2005) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | During the last decade, hepatocyte transplantation has been suggested as a safe and potentially effective clinical option for the treatment of acute or decompensating chronic liver failure as well as for hereditary liver disease. Currently, one of the major limiting factors for clinical application is the insufficient access to suitable liver cell preparations. In cooperation with the German and Catalane organ procurement organizations, a routine procedure for the isolation of hepatocytes from donor organs rejected for transplantation (n = 117) has been established. The process is performed according to the current EC Guidelines for Good Manufacturing Practice (cGMP) and all corresponding national laws and regulations concerning donor organ and tissue procurement. In about 50% of the cases (n = 58) the three-step perfusion procedure has been completed with an average total cell yield of 5.9 × 109 cells per organ, the cell preparations displaying a mean viability of 64%. The mean specific yield was 3.6 × 106 total and 2.6 × 106 viable cells per gram liver tissue, respectively. Specific cell yields from three infantile donor livers were considerably higher. No correlation between isolation efficiency and cold ischemia time or donor age was found within the adult organ donors. In contrast, organs with a severe steatosis generally did not result in successful cell isolation. Results of sterility and endotoxin determination are also presented. In summary, a standardized and cGMP conform method of hepatocyte isolation from nontransplantable liver organs was established, which reproducibly yields large amounts of hepatocytes suitable for therapeutic application. |
| Databáze: | OpenAIRE |
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