Glycerol kinase deficiency: Evidence for complexity in a single gene disorder
| Autor: | M Kimura, L L McCabe, Katrina M. Dipple, Vivian E. Shih, J Dallongeville, T Inokuchi, G O Roederer, Seiji Yamaguchi, H J Marx, Ichiro Yoshida, Edward R.B. McCabe, Bing-Ling Huang, Yao-Hua Zhang |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Models Molecular X Chromosome Genotype Protein Conformation DNA Mutational Analysis Mutation Missense Biology Contiguous gene syndrome Asymptomatic Cell Line Catalytic Domain Glycerol Kinase Genetics medicine Humans Missense mutation Genetics (clinical) Point mutation Chromosome Mapping Glycerol kinase deficiency medicine.disease Phenotype Hyperglycerolemia biology.protein medicine.symptom |
| Zdroj: | Human Genetics. 109:55-62 |
| ISSN: | 1432-1203 0340-6717 |
| Popis: | Glycerol kinase deficiency (GKD) occurs as part of an Xp21 contiguous gene syndrome or as isolated GKD. The isolated form can be either symptomatic with episodic metabolic and central nervous system (CNS) decompensation or asymptomatic with hyperglycerolemia and glyceroluria only. To better understand the pathogenesis of isolated GKD, we sought individuals with point mutations in the GK coding region and measured their GK enzyme activities. We identified six individuals with missense mutations: four (N288D, A305V, M428T, and Q438R) among males who were asymptomatic and two (D198G, R405Q) in individuals who were symptomatic. GK activity measured in lymphoblastoid cell lines or fibroblasts was similar for the symptomatic and the asymptomatic individuals. Mapping of the individuals' missense mutations to the three-dimensional structure of Escherichia coli GK revealed that the symptomatic individuals' mutations are in the same region as a subset of the mutations among the asymptomatic individuals, adjacent to the active-site cleft. We conclude that, like many other disorders, GK genotype does not predict GKD phenotype. We hypothesize that the phenotype of an individual with GKD is a complex trait influenced by additional, independently inherited genes. |
| Databáze: | OpenAIRE |
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