Murine AML12 hepatocytes allow Salmonella Typhimurium T3SS1-independent invasion and intracellular fate
| Autor: | Sylvie M. Roche, Julien Burlaud-Gaillard, Isabelle Virlogeux-Payant, Sébastien Holbert, Agnès Wiedemann, Stéphane Méresse, Philippe Velge, Sonia Georgeault, Emilie Barilleau, Jérôme Trotereau |
|---|---|
| Přispěvatelé: | Infectiologie et Santé Publique (UMR ISP), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme IBISA de Microscopie Electronique [CHRU de Tours] (UNIV Tours), Centre Hospitalier Régional Universitaire de Tours (CHRU TOURS)-Université de Tours, Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), INRAE, Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Université de Tours (UT), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), HOLBERT, Sébastien |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Salmonella
Cell signaling Cell biology Science [SDV]Life Sciences [q-bio] Vacuole medicine.disease_cause Microbiology Article Type three secretion system T3SS1 invasion factors 03 medical and health sciences Mice Bacterial Proteins medicine Type III Secretion Systems Animals Cytoskeleton 030304 developmental biology 0303 health sciences Multidisciplinary 030306 microbiology Effector Chemistry Pathogenicity island [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology [SDV] Life Sciences [q-bio] Salmonella Infections Vacuoles Salmonella Typhimurium Hepatocytes Medicine [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Intracellular |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-02054-z⟩ Scientific Reports, 2021, 11 (1), 16 p. ⟨10.1038/s41598-021-02054-z⟩ Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-02054-z⟩ |
| Popis: | Numerous studies have demonstrated the key role of the Salmonella Pathogenicity Island 1-encoded type III secretion system (T3SS1) apparatus as well as its associated effectors in the invasion and intracellular fate of Salmonella in the host cell. Several T3SS1 effectors work together to control cytoskeleton networks and induce massive membrane ruffles, allowing pathogen internalization. Salmonella resides in a vacuole whose maturation requires that the activity of T3SS1 subverts early stages of cell signaling. Recently, we identified five cell lines in which Salmonella Typhimurium enters without using its three known invasion factors: T3SS1, Rck and PagN. The present study investigated the intracellular fate of Salmonella Typhimurium in one of these models, the murine hepatocyte cell line AML12. We demonstrated that both wild-type Salmonella and T3SS1-invalidated Salmonella followed a common pathway leading to the formation of a Salmonella containing vacuole (SCV) without classical recruitment of Rho-GTPases. Maturation of the SCV continued through an acidified phase that led to Salmonella multiplication as well as the formation of a tubular network resembling Salmonella induced filaments (SIF). The fact that in the murine AML12 hepatocyte, the T3SS1 mutant induced an intracellular fate resembling to the wild-type strain highlights the fact that Salmonella Typhimurium invasion and intracellular survival can be completely independent of T3SS1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink