Identification of common antigens in Babesia bovis, B. bigemina, and B. divergens
| Autor: | B. Carcy, Eric Precigout, Julio V. Figueroa, André Gorenflot |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Immunoblotting
Molecular Sequence Data Antibodies Protozoan Babesia Cattle Diseases Antigens Protozoan Biology Cross Reactions Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Mice History and Philosophy of Science Antigen Complementary DNA Immunoscreening Babesiosis medicine Animals Immunoprecipitation Amino Acid Sequence Fluorescent Antibody Technique Indirect Phylogeny Gene Library Antiserum Base Sequence General Neuroscience Babesia bovis DNA Protozoan biology.organism_classification medicine.disease Virology Molecular Weight biology.protein Cattle Electrophoresis Polyacrylamide Gel Antibody Gerbillinae Sequence Alignment |
| Zdroj: | Annals of the New York Academy of Sciences. 1081 |
| ISSN: | 0077-8923 |
| Popis: | Bovine babesiosis, caused by Babesia bovis, B. bigemina, and B. divergens, is a significant impediment to livestock production in countries with tropical/subtropical and temperate climates. Previous studies conducted on the immunoprophylaxis against the disease and diagnosis of these parasites has demonstrated the presence of similar antigens. The objective of this article was to identify and partially characterize antigens conserved among these three species. Immunochemical analysis using sera from cattle immunized individually with antigens from these three Babesia species revealed a number of antigens recognized by heterologous antisera. Cross-reactions were more evident in sera from cattle immunized with B. bovis/B. bigemina which recognized several antigens (15 kDa to >200 kDa) in B. divergens. Immunoscreening of a B. divergens cDNA library with bovine serum to B. bigemina allowed the isolation of five clones and DNA sequencing of plasmid BdJF5 showed a 680 bp cDNA insert. Basic Local Alignment Search Tool (BLAST) analysis of the predicted amino acid sequence revealed 47% identity with a protein identified as αNAC.Serum from mice immunized with a recombinant Glutathione S-Transferase-BdJF5 fusion protein immunoprecipitated a 20 kDa B. bovis antigen. However, 30 kDa and 18 kDa antigens were immunoprecipitated from B. divergens and immunoblotting analysis revealed the recognition of a 35 kDa B. bigemina antigen. An indirect fluorescence antibody assay on merozoites showed strong reaction with B. divergens and weak recognition of B. bovis and B. bigemina. Despite the existent antigenic polymorphism among the Babesia spp., these results demonstrated that common antigens occur between European B. divergens and Mexican B. bovis/B. bigemina. |
| Databáze: | OpenAIRE |
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